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Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
IgE antibodies from schistosomiasis patients to recognize epitopes in potato apyrase
Michélia Antônia do Nascimento Gusmão1, Nayara Braga Emídio1, Danielle Gomes Marconato1
1Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brasil.
Introduction:
High percentages of structural identity and cross-immunoreactivity have been reported between potato apyrase and Schistosoma mansoni ATP diphosphohydrolase (SmATPDases) isoforms, showing the existence of particular epitopes shared between these proteins.
Methods:
Potato apyrase was employed using ELISA, western blot, and mouse immunization methods to verify IgE reactivity.
Results:
Most of the schistosomiasis patient's (75%) serum was seropositive for potato apyrase and this protein was recognized using western blotting, suggesting that parasite and plant proteins share IgE-binding epitopes. C57BL/6 mice immunized with potato apyrase showed increased IgE antibody production.
Conclusions:
Potato apyrase and SmATPDases have IgE-binding epitopes.
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