Jove
Visualize
Contact Us

Related Experiment Videos

Intermediate-filament expression in thyroid gland carcinomas.

S Schröder, B Dockhorn-Dworniczak, H Kastendieck

    Virchows Archiv. A, Pathological Anatomy and Histopathology
    |January 1, 1986
    PubMed
    Summary

    This study investigated intermediate-filament (IF) protein expression in thyroid carcinomas. Cytokeratins were common, while vimentin and neurofilaments showed varied expression, aiding in tumor classification.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Potential formation of PCDD/Fs in triclosan wastewater treatment: An overall toxicity assessment under a life cycle approach.

    The Science of the total environment·2019
    Same author

    International round-robin experiment for angle-resolved light scattering measurement.

    Applied optics·2019
    Same author

    FLASHForward: plasma wakefield accelerator science for high-average-power applications.

    Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2019
    Same author

    [Erratum to: Simulation training as part of clinical riskmanagement : A health economic view].

    Der Anaesthesist·2019
    Same author

    [Simulation training as part of clinical risk management : A health economic view].

    Der Anaesthesist·2019
    Same author

    Tunable Plasma-Based Energy Dechirper.

    Physical review letters·2019
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies

    Area of Science:

    • Oncology
    • Cell Biology
    • Immunohistochemistry

    Background:

    • Intermediate filaments (IFs) are crucial for cellular structure and function.
    • Differential expression of IF proteins can aid in classifying tumors, particularly in the thyroid.
    • Understanding IF protein profiles in thyroid carcinomas is essential for accurate diagnosis and understanding tumor heterogeneity.

    Purpose of the Study:

    • To immunohistochemically analyze the expression of cytokeratin, vimentin, and neurofilament proteins in various types of primary thyroid carcinomas.
    • To investigate the potential diagnostic value of IF protein expression patterns in differentiating thyroid tumor subtypes.
    • To explore the relationship between IF protein expression and the intrinsic heterogeneity of thyroid tumors.

    Main Methods:

    Related Experiment Videos

  • Immunohistological examination of 200 paraffin-embedded primary thyroid carcinoma specimens.
  • Analysis of cytokeratin, vimentin, and neurofilament protein expression using specific antibodies.
  • Double label immunofluorescence microscopy performed on snap-frozen tissue from 36 cases.

    • Main Results:

    • Cytokeratin reactivity was universally observed in follicular, papillary, and medullary carcinomas.
    • Vimentin expression was detected in a significant proportion of papillary, follicular, and medullary carcinomas.
    • Neurofilaments were found in approximately 13% of medullary tumors, often co-expressing vimentin. Anaplastic carcinomas showed variable cytokeratin and vimentin expression.

    Conclusions:

    • IF protein expression patterns, particularly cytokeratin and vimentin, differ among thyroid carcinoma subtypes.
    • The observed variations in IF protein expression suggest intrinsic heterogeneity within thyroid tumors, especially anaplastic types.
    • IF protein analysis holds diagnostic value for thyroid carcinoma classification and understanding tumor biology.