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Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
Published on: September 28, 2012
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TRAIP regulates Histone H2B monoubiquitination in DNA damage response pathways
Ye Gi Han1, Miyong Yun2, Minji Choi2
1Department of Biological Sciences, Sungkyunkwan University, Suwon 440‑746, Republic of Korea.
Oncology Reports
|April 4, 2019
Summary
Histone H2B monoubiquitination is downregulated in lung adenocarcinoma. Tumor necrosis factor receptor‑associated factor‑interacting protein (TRAIP) regulates this modification, and low levels of both correlate with poor patient survival.
Area of Science:
- Molecular Biology
- Oncology
- Epigenetics
Background:
- Histone H2B monoubiquitination is crucial for DNA damage response and linked to cancer.
- Its role in lung adenocarcinoma remains largely unexplored.
Purpose of the Study:
- To investigate the clinical significance of H2B monoubiquitination in lung adenocarcinoma.
- To explore the regulatory role of TRAIP in H2B monoubiquitination within this cancer type.
Main Methods:
- Immunohistochemical analysis of 68 lung adenocarcinoma patient samples.
- TRAIP depletion using siRNA and assessment of H2B monoubiquitination.
- Analysis of TRAIP deletion mutants and correlation with patient survival.
Main Results:
- H2B monoubiquitination was significantly reduced in lung adenocarcinoma tissues.
- TRAIP depletion diminished ionizing radiation-induced H2B monoubiquitination.
- Nuclear TRAIP expression positively correlated with H2B monoubiquitination; low levels of both predicted poor survival.
Conclusions:
- TRAIP is a novel regulator of H2B monoubiquitination in lung adenocarcinoma.
- TRAIP and H2B monoubiquitination levels are potential prognostic biomarkers for lung adenocarcinoma.
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