Related Experiment Video

Updated: Jan 26, 2026

A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro
10:36

A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro

Published on: January 9, 2018

7.6K

Natural killer cell monitoring in cutaneous melanoma - new dynamic biomarker

Gheorghița Isvoranu1, Mihaela Surcel2,3, Radu-Ionuț Huică2,4

  • 1Animal Husbandry, 'Victor Babeș' National Institute of Pathology, 050096 Bucharest, Romania.

Oncology Letters
|April 5, 2019
PubMed

Insights

In melanoma-bearing mice, Natural Killer (NK) cells in the spleen were reduced in number and altered in phenotype, suggesting potential tumor growth promotion. This impacts understanding of antitumor immunity and NK cell-based therapies.

Area of Science:

  • Immunology
  • Oncology
  • Cancer Research

Background:

  • Melanoma causes most skin cancer deaths.
  • The immune system regulates tumor cell proliferation.
  • Murine cancer models enhance understanding of immune responses in malignancies.

Purpose of the Study:

  • Investigate T-CD4+, T-CD8+, B lymphocytes, and NK cells in melanoma-bearing mice versus controls.
  • Assess potential for tumor growth-promoting immunosuppression.
  • Examine changes in immune cell populations in a melanoma mouse model.

Main Methods:

  • Collected peripheral blood and spleen cells from melanoma-bearing mice and healthy controls.
  • Analyzed percentages and phenotypes of T lymphocytes (CD4+, CD8+), B lymphocytes, and NK cells.
  • Utilized flow cytometry for immune cell characterization.

Main Results:

  • Reduced percentage of NK cells observed in the spleen of melanoma-bearing mice.
  • Significant differences in NK cell phenotype compared to control mice.
  • T-CD4+, T-CD8+, and B lymphocyte populations were also assessed for changes.

Conclusions:

  • Melanoma in mice is associated with reduced splenic NK cell numbers and altered NK cell phenotype.
  • These immune alterations may contribute to tumor growth-promoting immunosuppression.
  • Findings highlight the importance of NK cell status in melanoma and for developing NK-based therapies.
Keywords:
B-lymphocytesNK cellsT-lymphocytesmelanomatumor-bearing mice

More Related Videos

Analysis of Human Natural Killer Cell Metabolism09:03

Analysis of Human Natural Killer Cell Metabolism

Published on: June 22, 2020

7.4K
Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies09:54

Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies

Published on: May 22, 2020

3.9K

Related Concept Videos

What is Natural Selection?01:32

What is Natural Selection?

Natural selection is an evolutionary process in which individuals with survival-promoting traits reproduce at higher rates. These favorable traits become more common within a population or species. Naturally selected traits initially arise via random genetic mutations. In order for selection to occur, there must be variation within a population, the trait controlling the variation must be heritable, and there must be an evolutionary advantage for variation in the trait.
126.6K
Nature and Nurture01:10

Nature and Nurture

Many human characteristics, like height, are shaped by both nature—in other words, by our genes—and by nurture, or our environment. For example, chronic stress during childhood inhibits the production of growth hormones and consequently reduces bone growth and height. Scientists estimate that 70-90% of variation in height is due to genetic differences among individuals, and 10-30% of variation in height is due to differences in the environments that individuals experience,...
22.2K
Dynamic Equilibrium02:20

Dynamic Equilibrium

A reversible chemical reaction represents a chemical process that proceeds in both forward (left to right) and reverse (right to left) directions. When the rates of the forward and reverse reactions are equal, the concentrations of the reactant and product species remain constant over time and the system is at equilibrium. A special double arrow is used to emphasize the reversible nature of the reaction. The relative concentrations of reactants and products in equilibrium systems vary greatly;...
62.1K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.6K
Chirality in Nature02:30

Chirality in Nature

Chirality is the most intriguing yet essential facet of nature, governing life’s biochemical processes and precision. It can be observed from a snail shell pattern in a macroscopic world to an amino acid, the minutest building block of life. Most of the snails around the world have right-coiled shells because of the intrinsic chirality in their genes. All the amino acids present in the human body exist in an enantiomerically pure state, except for glycine - the sole achiral amino acid.
17.0K
The Wave Nature of Light02:12

The Wave Nature of Light

The nature of light has been a subject of inquiry since antiquity. In the seventeenth century, Isaac Newton performed experiments with lenses and prisms and was able to demonstrate that white light consists of the individual colors of the rainbow combined together. Newton explained his optics findings in terms of a "corpuscular" view of light, in which light was composed of streams of extremely tiny particles traveling at high speeds according to Newton's laws of motion.
61.1K