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Published on: July 3, 2025
Formate Oxidase (FOx) from Aspergillus oryzae: One Catalyst Enables Diverse H2 O2 -Dependent Biocatalytic Oxidation
Florian Tieves1, Sébastien Jean-Paul Willot1, Morten Martinus Cornelis Harald van Schie1
1Department of Biotechnology, University of Technology Delft, van der Massweg 9, 2629HZ, Delft, The Netherlands.
Abstract:
An increasing number of biocatalytic oxidation reactions rely on H2 O2 as a clean oxidant. The poor robustness of most enzymes towards H2 O2 , however, necessitates more efficient systems for in situ H2 O2 generation. In analogy to the well-known formate dehydrogenase to promote NADH-dependent reactions, we here propose employing formate oxidase (FOx) to promote H2 O2 -dependent enzymatic oxidation reactions. Even under non-optimised conditions, high turnover numbers for coupled FOx/peroxygenase catalysis were achieved.
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