Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Kinetic Energy00:23

Kinetic Energy

43.2K
Kinetic energy is the ability of an object in motion to do work or enact change. It can take on many forms. For instance, water flowing down a waterfall has kinetic energy. In biological systems, particles of light travel and are absorbed by plants to create chemical energy. Animals consume the chemical energy and give off molecules that carry their scent through the air. They also generate kinetic energy when they run away from predators. Entire systems also possess kinetic energy, like the...
43.2K
Enzyme Kinetics01:19

Enzyme Kinetics

103.9K
Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
103.9K
Kinetic Molecular Theory: Molecular Velocities, Temperature, and Kinetic Energy03:07

Kinetic Molecular Theory: Molecular Velocities, Temperature, and Kinetic Energy

29.8K
The kinetic molecular theory qualitatively explains the behaviors described by the various gas laws. The postulates of this theory may be applied in a more quantitative fashion to derive these individual laws.
29.8K
Elimination Kinetics: First-Order and Zero-Order01:05

Elimination Kinetics: First-Order and Zero-Order

2.8K
Eliminating drugs from the body is a vital process that occurs through excretion or metabolism. Understanding the kinetics of drug elimination is crucial for drug development, dosage determination, and optimizing patient outcomes.
Drug clearance depends on the rate of drug elimination and its plasma concentration. Another important parameter is a drug's half-life, which is the time required for its concentration to decrease by half. In most cases, drug clearance follows first-order...
2.8K
Kinetic Friction01:26

Kinetic Friction

1.4K
Consider a truck trying to pull a stationary car. As the truck exerts a force on the car, static friction is created at the point of contact between the two surfaces. This frictional force resists the car's movement and keeps it at rest. However, when the applied force by the truck surpasses the limiting static frictional force, an interesting phenomenon occurs. The frictional force at the interface reduces to a lower value, known as the kinetic frictional force. At this point, the car...
1.4K
Kinetic Energy - I01:18

Kinetic Energy - I

12.5K
It’s plausible to suppose that the greater the velocity of a body, the greater effect it could have on other bodies. This does not depend on the direction of the velocity, only its magnitude. At the end of the seventeenth century, a quantity was introduced into mechanics to explain collisions between two perfectly elastic bodies, in which one body makes a head-on collision with an identical body at rest. When they collide, the first body stops, and the second body moves off with the...
12.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Computational Modeling of ADLumin Chemiluminescence: Oxygenation and Dioxetanone Formation.

Journal of computational chemistry·2026
Same author

Towards non-blinking and photostable perovskite quantum dots.

Nature communications·2025
Same author

Biexciton-like Auger Blinking in Strongly Confined CsPbBr

The journal of physical chemistry letters·2023
Same author

Amine Functionalization of Silica Sol-Gel Thin Films via Kinetic Doping: A Novel, Green Approach.

ACS omega·2019
Same author

Kinetically Doped Silica Sol-Gel Optical Biosensors: Expanding Potential Through Dip-Coating.

ACS omega·2018
Same author

Silica Sol-Gel Optical Biosensors: Ultrahigh Enzyme Loading Capacity on Thin Films via Kinetic Doping.

The journal of physical chemistry. B·2017

Related Experiment Video

Updated: Jan 26, 2026

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
15:27

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

21.5K

Multienzyme, Multistep Biosensor Produced through Kinetic Doping.

Matthew S Crosley1, Wai Tak Yip1

  • 1Department of Chemistry and Biochemistry , University of Oklahoma , Norman , Oklahoma 73019-5251 , United States.

The Journal of Physical Chemistry. B
|April 5, 2019
PubMed
Summary

This study introduces kinetic doping for creating multi-enzyme biosensors. This novel method efficiently loads enzymes for detecting glucose, simplifying biosensor production.

More Related Videos

Monolayer Contact Doping of Silicon Surfaces and Nanowires Using Organophosphorus Compounds
09:45

Monolayer Contact Doping of Silicon Surfaces and Nanowires Using Organophosphorus Compounds

Published on: December 2, 2013

7.9K
An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
09:33

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

Published on: December 17, 2018

10.7K

Related Experiment Videos

Last Updated: Jan 26, 2026

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
15:27

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

21.5K
Monolayer Contact Doping of Silicon Surfaces and Nanowires Using Organophosphorus Compounds
09:45

Monolayer Contact Doping of Silicon Surfaces and Nanowires Using Organophosphorus Compounds

Published on: December 2, 2013

7.9K
An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
09:33

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

Published on: December 17, 2018

10.7K

Area of Science:

  • Biochemistry
  • Biosensor Technology
  • Materials Science

Background:

  • Kinetic doping is a new technique for enzyme loading in biosensors.
  • Previous applications focused on single enzymes.
  • Multistep reaction pathways offer enhanced detection capabilities.

Purpose of the Study:

  • To demonstrate kinetic doping for biosensors with multiple enzymes.
  • To utilize a multistep reaction pathway for glucose detection.
  • To evaluate the efficiency and ease of production of dual-enzyme biosensors.

Main Methods:

  • Enzyme loading using kinetic doping with glucose oxidase (GOD) and horseradish peroxidase (HRP).
  • Fabrication of dual-enzyme thin films.
  • Quantification of enzyme loading and activity.
  • Assessment of glucose detection in solution.

Main Results:

  • Successfully loaded both GOD and HRP into thin films using kinetic doping.
  • Achieved high loading efficiencies: 33% for HRP and 92% for GOD.
  • Demonstrated effective glucose detection via tandem enzyme action.
  • Quantified enzyme concentrations: 1.8 ± 0.1 mmol/L HRP and 0.22 ± 0.01 mmol/L GOD.

Conclusions:

  • Kinetic doping enables efficient co-loading of multiple enzymes for biosensor applications.
  • The developed dual-enzyme biosensor is effective for glucose detection.
  • The process is simple, protein-friendly, and facilitates the creation of complex biosensors.