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Updated: Jan 26, 2026

Conducting Maximal and Submaximal Endurance Exercise Testing to Measure Physiological and Biological Responses to Acute Exercise in Humans
Published on: October 17, 2018
Laboratory Validation of Hexoskin Biometric Shirt at Rest, Submaximal Exercise, and Maximal Exercise While Riding a
Cara M Smith1, Steven N Chillrud, Darby W Jack
1Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York, New York (Ms Smith, Dr Jack); Department of Geochemistry, Lamont-Doherty Earth Observatory, Columbia University, Palisades, New York (Dr Chillrud, Dr Yang); Department of Environmental Health, Boston University School of Public Health, Boston, Massachusetts (Dr Kinney); and Division of Pulmonary, Allergy, and Critical Care Medicine, Department of Medicine, Columbia University Medical Center (Dr Layton), New York, New York.
Objective:
Evaluate Hexoskin performance on a stationary bike against "gold standard" laboratory equipment and develop adjustment models for future use in field settings.
Methods:
Compared respiratory rate (RR), tidal volume (VT), minute ventilation (VE), and heart rate (HR) measured by the Hexoskin shirt to simultaneous spirometry and full 12-lead electrocardiogram during a laboratory based incremental exercise test on a stationary bicycle.
Results:
Data from 17 participants demonstrated Hexoskin VT and VE had the best agreement in the submaximal exercise level (discrepancies less than or equal to 5.3%) with larger discrepancies observed at rest (less than or equal to 15.3%) and at maximal exercise level (less than or equal to 11.7%). The discrepancies for HR and RR were lower at all levels (less than 10%). Adjusting for sex and body weight allowed for a single VE algorithm across the entire range of effort (r = 0.89).
Conclusion:
These discrepancies are acceptable for field use in comparison to the ranges typical of bicycle commuting.
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