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Loc108167440 suppressed myeloma cell growth by P53-mediated apoptosis

Bing Zhai1,2, Chunmei Hou2, Ruonan Xu2,3

  • 1Department of Geriatric Hematology, Chinese PLA General Hospital National Clinical Research Center for Geriatric Diseases , Beijing , China.

Leukemia & Lymphoma
|April 6, 2019
PubMed

Insights

Overexpressing Loc108167440, a novel molecule, suppressed multiple myeloma (MM) cell growth and tumor progression by inducing p53-mediated apoptosis. This finding suggests Loc108167440 as a potential therapeutic target for MM.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Multiple myeloma (MM) is characterized by the uncontrolled proliferation of cancerous plasma cells (PC).
  • Identifying novel molecular targets that distinguish MM PC from healthy PC is crucial for developing effective MM therapies.
  • Current therapeutic strategies aim to specifically target and eliminate MM PC.

Purpose of the Study:

  • To identify novel genes and molecules that are differentially expressed between MM PC and normal PC.
  • To investigate the functional role of Loc108167440 in MM cell growth and tumor progression.
  • To explore the potential of Loc108167440 as a therapeutic agent for multiple myeloma.

Main Methods:

  • RNA sequencing was employed to compare gene expression profiles between LPS-induced plasmablasts/PC and the SP 2/0 myeloma cell line.
  • Loc108167440 was overexpressed in SP 2/0 cells to assess its impact on cell viability and apoptosis.
  • A xenograft mouse model was used to evaluate the effect of Loc108167440 overexpression on tumor progression.
  • Western blotting or similar techniques were used to analyze p53 expression levels.

Main Results:

  • SP 2/0 myeloma cells exhibited significantly lower levels of Loc108167440 mRNA compared to LPS-induced PB/PC.
  • Overexpression of Loc108167440 led to a reduction in SP 2/0 cell number by inducing apoptotic cell death.
  • Loc108167440 overexpression suppressed tumor growth in a xenograft mouse model of MM.
  • Loc108167440 overexpression was found to up-regulate the expression of p53 in SP 2/0 cells.

Conclusions:

  • Loc108167440 plays a significant role in suppressing the growth of multiple myeloma cells.
  • The anti-myeloma effect of Loc108167440 is mediated through the induction of p53-dependent apoptosis.
  • Loc108167440 represents a promising novel therapeutic target for the treatment of multiple myeloma.
Keywords:
Loc108167440P53SP 2/0 cellsmultiple myelomaplasma cells

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