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Updated: Jan 26, 2026
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Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
Published on: November 24, 2010
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MoS2 flakes stabilized with DNA/RNA nucleotides: In vitro cell response.
M Cicuéndez1, V S Silva2, J Santos2
1CICECO- Aveiro Institute of Materials, Chemistry Department, University of Aveiro (UA), Campus Universitário de Santiago, 3810-193 Aveiro, Portugal; NRG-TEMA, Mechanical Engineering Department, University of Aveiro (UA), Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
Summary
Nucleotide-stabilized molybdenum disulfide (MoS2) nanomaterials show excellent biocompatibility and cellular uptake. These MoS2 flakes are promising for drug/gene delivery and cancer therapy applications.
Area of Science:
- Materials Science
- Nanotechnology
- Biomedical Engineering
Background:
- Two-dimensional transition metal dichalcogenides (TMDCs) like MoS2 are emerging as nanomaterials for biomedical applications.
- Effective interaction with biological systems requires surface functionalization with biomolecules.
- Nucleotides offer a strong Lewis acid-base interaction for stabilizing MoS2 flakes.
Purpose of the Study:
- To functionalize MoS2 flakes with nucleotides (AMP, GMP, FMN) for improved biocompatibility.
- To evaluate the impact of nucleotide-stabilized MoS2 on cell viability, proliferation, ROS production, and osteoblast differentiation.
- To assess the cellular incorporation and intracellular localization of these nanomaterials.
Main Methods:
- Stabilization of MoS2 flakes using adenosine monophosphate (AMP), guanosine monophosphate (GMP), and flavin mononucleotide (FMN).
- Assessment of cytotoxicity, intracellular reactive oxygen species (ROS) production, and preosteoblast differentiation in MC3T3-E1 and Saos-2 cells.
- Evaluation of nanomaterial incorporation and intracellular localization using cell imaging techniques.
Main Results:
- Nucleotide-stabilized MoS2 flakes demonstrated excellent biocompatibility.
- No adverse effects on cell plasma membrane integrity were observed.
- Differential in vitro cellular responses were noted between tumor and undifferentiated cells.
Conclusions:
- Nucleotide-stabilized MoS2 nanomaterials are suitable for drug/gene delivery due to their biocompatibility and cellular uptake.
- The distinct cellular responses suggest potential applications in targeted cancer therapy.
- Further research into TMDCs functionalized with biomolecules holds promise for advanced biomedical applications.
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