Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

18.7K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
18.7K
Replication in Eukaryotes02:31

Replication in Eukaryotes

204.3K
Overview
204.3K
Replication in Eukaryotes01:29

Replication in Eukaryotes

17.3K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
17.3K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

26.8K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
26.8K
The Tree of Life - Bacteria, Archaea, Eukaryotes02:40

The Tree of Life - Bacteria, Archaea, Eukaryotes

38.1K
The “tree of life” describes the evolution of life and the evolutionary relationships between organisms. The root of the tree is the common ancestor to all life on Earth. All other species radiate from this point, much like the branches of a tree. The numerous tips of these branches on the tree of life represent every living, or extant, species. Extinct species, which are species that no longer exist, can be found towards the center of the tree. Currently, these organisms, both...
38.1K
Eukaryotic Evolution01:24

Eukaryotic Evolution

40.4K
The endosymbiont theory is the most widely accepted theory of eukaryotic evolution; however, its progression is still somewhat debated. According to the nucleus-first hypothesis, the ancestral prokaryote first evolved a membrane to enclose DNA and form the nucleus. Conversely, the mitochondria-first hypothesis suggests that the nucleus was formed after endosymbiosis of mitochondria.
Contrary to the endosymbiont theory, the eukaryote-first hypothesis proposes that the simpler prokaryotic and...
40.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Biobank Rare Variant consortium powers the discovery of rare genetic associations through global collaboration.

medRxiv : the preprint server for health sciences·2026
Same author

Diffusional aging at water/oil interfaces laden with charged nanoparticles studied by single-molecule tracking.

Nature communications·2026
Same author

Strength-Toughness-Wear Coupling Mechanisms of Low-Carbon Martensitic Wear-Resistant Steel Enabled by Ti/Nb Microalloying-Driven Carbide Precipitation and Synergistic Regulation of Tempered Microstructures.

Materials (Basel, Switzerland)·2026
Same author

RBM43 promotes glioma aggressiveness and shapes the immune microenvironment through macrophage polarization.

Discover oncology·2026
Same author

Integrated Network Pharmacology, Molecular Docking and Experimental Validation Reveal That Quercetin Suppresses Clear Cell Renal Cell Carcinoma via MMP9-Associated Macrophage Polarization.

Biomedicines·2026
Same author

Radiopharmaceutical-Guided Personalized Adjuvant Radiotherapy Planning after Esophageal Cancer Resection.

Cancer biotherapy & radiopharmaceuticals·2026
Same journal

conMItion: an R package adjusting confounding factors for associations in multi-omics.

Bioinformatics (Oxford, England)·2026
Same journal

SpaMFG: a Spatial Multi-omics Integration Method based on Feature Grouping.

Bioinformatics (Oxford, England)·2026
Same journal

CSCN: Inference of Cell-Specific Causal Networks Using Single-Cell RNA-Seq Data.

Bioinformatics (Oxford, England)·2026
Same journal

Sparse CCA-Based Mediation Analysis with High-Dimensional Exposures and Mediators.

Bioinformatics (Oxford, England)·2026
Same journal

Enhancing Cross-Context Generalization in Drug Perturbation Prediction with a Multimodal Conditional Diffusion Framework.

Bioinformatics (Oxford, England)·2026
Same journal

Primer Design through Submodular Function Estimation.

Bioinformatics (Oxford, England)·2026
See all related articles

Related Experiment Video

Updated: Jan 26, 2026

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
07:38

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Published on: April 11, 2019

13.4K

IntronDB: a database for eukaryotic intron features.

Dapeng Wang1,2

  • 1Department of Plant Sciences, University of Oxford, Oxford, UK.

Bioinformatics (Oxford, England)
|April 6, 2019
PubMed
Summary
This summary is machine-generated.

IntronDB catalogs 50 million spliceosomal introns from over 1000 eukaryotic genomes, enabling evolutionary analysis of intron dynamics and features across species. This resource facilitates comparisons between introns and exons, aiding in understanding gene structure evolution.

More Related Videos

Generation of Comprehensive Thoracic Oncology Database - Tool for Translational Research
11:18

Generation of Comprehensive Thoracic Oncology Database - Tool for Translational Research

Published on: January 22, 2011

16.5K
A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

2.4K

Related Experiment Videos

Last Updated: Jan 26, 2026

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
07:38

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Published on: April 11, 2019

13.4K
Generation of Comprehensive Thoracic Oncology Database - Tool for Translational Research
11:18

Generation of Comprehensive Thoracic Oncology Database - Tool for Translational Research

Published on: January 22, 2011

16.5K
A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

2.4K

Area of Science:

  • Genomics
  • Bioinformatics
  • Evolutionary Biology

Background:

  • Eukaryotic intron evolution is dynamic, influenced by selective pressures and gene expression.
  • Limited knowledge exists regarding conserved splicing signals and regulatory elements within introns.
  • Understanding intron evolutionary trajectories requires comprehensive genomic data analysis.

Purpose of the Study:

  • To establish IntronDB, a unified framework for tracing spliceosomal intron evolutionary trajectories.
  • To catalog and characterize introns across major eukaryotic clades.
  • To enable comparative analyses of intronic and exonic features.

Main Methods:

  • Cataloged approximately 50 million introns from over 1000 eukaryotic genomes.
  • Categorized introns based on position (5'UTR, CDS, 3'UTR) and CDS intron phase (0, 1, 2).
  • Characterized intronic parameters (number, size, sequence composition, positioning) and compared them with exonic features.

Main Results:

  • IntronDB provides quality evaluation and detailed characterization of intronic and exonic features.
  • Analysis of dinucleotides around intron boundaries and consensus sequence features for different intron sizes.
  • Facilitated genome-wide statistical analysis of intronic features within and across genomes.

Conclusions:

  • IntronDB serves as a valuable resource for studying intron evolution and comparative genomics.
  • The database enables detailed analysis of intron dynamics and features across diverse eukaryotic lineages.
  • Facilitates future research into the functional and evolutionary roles of introns.