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Identification of lncRNA-miRNA-mRNA regulatory network associated with epithelial ovarian cancer cisplatin-resistant

Xin Zhao1, Dong-Yang Tang2, Xu Zuo1

  • 1Department of Pharmacy, Xinxiang Central Hospital, Xinxiang, Henan, P. R. China.

Journal of Cellular Physiology
|April 6, 2019
PubMed

Insights

This study identifies key genes and regulatory networks involved in cisplatin resistance in epithelial ovarian cancer (EOC). The findings offer insights into the molecular mechanisms driving drug resistance in EOC.

Area of Science:

  • Genomics
  • Molecular Biology
  • Oncology

Background:

  • Epithelial ovarian cancer (EOC) often develops resistance to cisplatin chemotherapy.
  • Understanding the molecular mechanisms of cisplatin resistance is crucial for improving treatment outcomes.

Purpose of the Study:

  • To construct a long noncoding RNA (lncRNA)-microRNA (miRNA)-messenger RNA (mRNA) regulatory network associated with cisplatin resistance in EOC.
  • To identify differentially expressed genes (DEGs), lncRNAs (DELs), and miRNAs (DEMs) in cisplatin-resistant EOC cells.

Main Methods:

  • Differential gene, lncRNA, and miRNA expression analysis between cisplatin-resistant and sensitive cell lines.
  • Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.
  • Construction of protein-protein interaction (PPI) networks and lncRNA-miRNA-mRNA regulatory networks using databases like miRTarBase and DIANA-LncBase.

Main Results:

  • Identified 485 significant DEGs, 85 DELs, and 5 DEMs.
  • Constructed a PPI network revealing 25 hub genes critical for cisplatin resistance.
  • Established a lncRNA-miRNA-mRNA regulatory network comprising 4 lncRNAs, 4 miRNAs, and 35 mRNAs.

Conclusions:

  • The study elucidates crucial gene alterations and regulatory networks implicated in EOC cisplatin resistance.
  • These findings contribute to understanding the molecular basis of drug resistance and may guide future therapeutic strategies.
Keywords:
cisplatin-resistantepithelial ovarian cancerlncRNAmRNAmiRNA

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