Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ionic Crystal Structures02:42

Ionic Crystal Structures

16.9K
Ionic crystals consist of two or more different kinds of ions that usually have different sizes. The packing of these ions into a crystal structure is more complex than the packing of metal atoms that are the same size.
Most monatomic ions behave as charged spheres, and their attraction for ions of opposite charge is the same in every direction. Consequently, stable structures for ionic compounds result (1) when ions of one charge are surrounded by as many ions as possible of the opposite...
16.9K
Biosynthesis of Polysaccharides01:26

Biosynthesis of Polysaccharides

594
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
594
Reproductive Cloning01:27

Reproductive Cloning

32.7K
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...
32.7K
Crystal Field Theory - Octahedral Complexes02:58

Crystal Field Theory - Octahedral Complexes

30.8K
Crystal Field Theory
To explain the observed behavior of transition metal complexes (such as colors), a model involving electrostatic interactions between the electrons from the ligands and the electrons in the unhybridized d orbitals of the central metal atom has been developed. This electrostatic model is crystal field theory (CFT). It helps to understand, interpret, and predict the colors, magnetic behavior, and some structures of coordination compounds of transition metals.
CFT focuses on...
30.8K
Cellulose and Pectic Polysaccharides01:15

Cellulose and Pectic Polysaccharides

4.7K
 Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth.  Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the...
4.7K
Crystal Growth: Principles of Crystallization01:25

Crystal Growth: Principles of Crystallization

4.9K
Crystallization is a phase transformation process in which crystals are precipitated from a supersaturated solution or formed from other sources. During crystallization, atoms or molecules arrange themselves into a well-defined, rigid crystal lattice to minimize energy.
Initiating crystallization involves manipulating the concentration of the solute and the temperature of the solution. Since crystal growth occurs when the ratio of concentration and solubility of the solute in the solvent...
4.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic Mapping of the 22q11.2 Deletion Syndrome (DiGeorge Syndrome) Microdeletion Types Revealed Novel Candidate Breakpoints.

Genes·2026
Same author

<i>APOE</i> Genotyping in Cognitive Disorders: Preliminary Observations from the Greek Population.

International journal of molecular sciences·2025
Same author

Decoding Non-Coding RNA Regulators in DITRA: From Genomic Insights to Potential Biomarkers and Therapeutic Targets.

Genes·2025
Same author

The Genetic Background of Ankylosing Spondylitis Reveals a Distinct Overlap with Autoimmune Diseases: A Systematic Review.

Journal of clinical medicine·2025
Same author

Enhanced Homogeneous Photocatalytic Hydrogen Evolution in a Binuclear Bio-Inspired Ni-Ni Complex Bearing Phenanthroline and Sulfidophenolate Ligands.

Chemistry (Weinheim an der Bergstrasse, Germany)·2025
Same author

Neuronal nicotinic acetylcholine receptor antibodies in autoimmune central nervous system disorders.

Frontiers in immunology·2024

Related Experiment Video

Updated: Jan 26, 2026

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

30.6K

The putative polysaccharide deacetylase Ba0331: cloning, expression, crystallization and structure determination.

Athena Andreou1, Petros Giastas1, Sofia Arnaouteli2

  • 1Department of Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.

Acta Crystallographica. Section F, Structural Biology Communications
|April 6, 2019
PubMed
Summary

The crystal structure of Bacillus anthracis polysaccharide deacetylase (Ba0331) was determined. This enzyme is crucial for anthrax bacterium survival and cell shape maintenance.

Keywords:
Ba0331Bacillus anthracisCE4 familyX-ray crystal structurelipoproteinmetalloenzymespolysaccharide deacetylase

More Related Videos

From Constructs to Crystals &#8211; Towards Structure Determination of &#946;-barrel Outer Membrane Proteins
09:55

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

Published on: July 4, 2016

14.0K
A Guide to Production, Crystallization, and Structure Determination of Human IKK1/&#945;
11:27

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Published on: November 2, 2018

9.5K

Related Experiment Videos

Last Updated: Jan 26, 2026

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

30.6K
From Constructs to Crystals &#8211; Towards Structure Determination of &#946;-barrel Outer Membrane Proteins
09:55

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

Published on: July 4, 2016

14.0K
A Guide to Production, Crystallization, and Structure Determination of Human IKK1/&#945;
11:27

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Published on: November 2, 2018

9.5K

Area of Science:

  • Structural biology
  • Microbiology
  • Biochemistry

Background:

  • Bacillus anthracis causes anthrax and possesses unique survival mechanisms.
  • Ba0331 is a key enzyme involved in bacterial adaptation and cell shape maintenance.
  • Understanding Ba0331's structure is vital for deciphering its function.

Purpose of the Study:

  • To determine the crystal structure of Ba0331.
  • To analyze the structural features of Ba0331, including its domains.
  • To investigate the structural plasticity of the fibronectin type 3-like domain.

Main Methods:

  • X-ray crystallography was used to determine the Ba0331 structure at 2.6 Å resolution.
  • Comparative structural analysis was performed on the identified domains.
  • Bioinformatic tools were utilized for domain identification and comparison.

Main Results:

  • The crystal structure of Ba0331 revealed a two-domain architecture.
  • The enzyme comprises a fibronectin type 3-like (Fn3-like) domain and a NodB catalytic domain.
  • The Fn3-like domain exhibits structural plasticity while maintaining conserved characteristics.

Conclusions:

  • The determined structure provides insights into the molecular mechanisms of Ba0331.
  • The structural plasticity of the Fn3-like domain may contribute to Ba0331's adaptive functions.
  • This study lays the foundation for further functional and therapeutic investigations of Ba0331.