Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Intrinsically Disordered Proteins02:18

Intrinsically Disordered Proteins

19.3K
Intrinsically disordered proteins are a group of proteins that do not fold into specific three-dimensional structures. Their structural flexibility allows them to complement ordered proteins to perform functions that are inaccessible to rigid structures. They are more common in eukaryotes than prokaryotes and may either be exclusively intrinsically disordered or hybrid proteins, consisting of a mix of ordered and disordered regions. The absence of a rigid structure in these proteins can be...
19.3K
Intrinsically Disordered Proteins02:18

Intrinsically Disordered Proteins

2.8K
2.8K
The Resting Membrane Potential01:21

The Resting Membrane Potential

142.1K
Overview
142.1K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

8.4K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
8.4K
Permeability of Concrete01:25

Permeability of Concrete

498
Permeability in the context of concrete refers to how easily liquids or gases can pass through the material. This quality is crucial for assessing the water-tightness and durability of concrete structures and their resistance to chemical attacks. Concrete permeability can be determined through comparative laboratory tests. These tests typically involve sealing a concrete specimen from the sides, applying water pressure to the top surface with pressure, and measuring the amount of water passing...
498
Extrinsic and Intrinsic Pathways of Hemostasis01:20

Extrinsic and Intrinsic Pathways of Hemostasis

12.5K
Blood clotting or coagulation involves extrinsic and intrinsic pathways, which ultimately merge into the common pathway, forming a fibrin clot.
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which...
12.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Glycolipids slow interfacial proton migration while preserving surface proton retention.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Evaluating Force Matching as a Parametrization Strategy for the CHARMM36m Force Field Using Phosphorylation.

The journal of physical chemistry. B·2026
Same author

A Semi-Quantitative Yeast Complementation Platform for Characterizing Urea and Ammonia Transport by Membrane Channels.

Current protocols·2026
Same author

Optical control of carrier-mediated ion transport by photoswitchable lipids.

Nanoscale·2025
Same author

Cysteine-mediated structural stabilization of the tetrameric GlpF.

European biophysics journal : EBJ·2025
Same author

Molecular Interactions within Nanoconfinement of Model DNA Nanostructures Controlled by Compensatory Kinetics as Revealed by Single-Molecule Fluorescence Analysis.

JACS Au·2025

Related Experiment Video

Updated: Jan 26, 2026

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates
10:33

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

Published on: February 23, 2018

26.0K

Intrinsic Membrane Permeability to Small Molecules.

Christof Hannesschlaeger1, Andreas Horner1, Peter Pohl1

  • 1From the Institute of Biophysics , Johannes Kepler University Linz , Gruberstrasse 40 , 4020 Linz , Austria.

Chemical Reviews
|April 6, 2019
PubMed
Summary
This summary is machine-generated.

Spontaneous molecule movement across membranes is crucial for life. New insights reveal a local partition coefficient, influenced by membrane depth and composition, governs this transport, refining the solubility-diffusion model.

More Related Videos

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
10:43

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

Published on: July 19, 2022

2.9K
Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery
12:48

Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery

Published on: September 12, 2015

10.1K

Related Experiment Videos

Last Updated: Jan 26, 2026

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates
10:33

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

Published on: February 23, 2018

26.0K
Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
10:43

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

Published on: July 19, 2022

2.9K
Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery
12:48

Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery

Published on: September 12, 2015

10.1K

Area of Science:

  • Biophysics
  • Membrane Transport
  • Physical Chemistry

Background:

  • Solute and solvent permeation across biological membranes is fundamental for physiological processes.
  • The solubility-diffusion model, established by Meyer and Overton, links substance solubility to membrane permeability.
  • Discrepancies in the model often arise from measurement methodologies, particularly concerning membrane CO2 transport.

Purpose of the Study:

  • To analyze the interplay of diffusion and chemical reactions in membrane transport.
  • To investigate solvent transport, weak acid permeation, and enzymatic reactions near membranes.
  • To refine the understanding of spontaneous membrane translocation for charged and uncharged molecules.

Main Methods:

  • Utilized scanning electrochemical microscopy (SECM) to visualize solute concentration gradients near planar membranes.
  • Analyzed diffusion-chemical reaction interplay for various transport scenarios.
  • Evaluated energetic contributions to charged species translocation.

Main Results:

  • Demonstrated that buffer reactions are critical for accurate spectroscopic studies of weak acid transport in vesicular suspensions.
  • Showed compatibility of membrane current with the solubility-diffusion model for charged species.
  • Identified a local partition coefficient, dependent on membrane penetration depth, as a key determinant of membrane translocation.

Conclusions:

  • Spontaneous membrane translocation of molecules is governed by a local partition coefficient, not solely organic phase solubility.
  • Factors such as cholesterol concentration and intrinsic membrane potentials influence this local partition coefficient.
  • The refined model provides a more comprehensive understanding of membrane transport mechanisms.