Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromosome Structure02:40

Chromosome Structure

26.0K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
26.0K
Chromosome Structure02:40

Chromosome Structure

6.2K
6.2K
Polytene Chromosomes02:04

Polytene Chromosomes

10.9K
Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also...
10.9K
Lampbrush Chromosomes01:51

Lampbrush Chromosomes

8.6K
In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...
8.6K
Chromosome Replication02:31

Chromosome Replication

10.5K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
10.5K
Chromosomal Theory of Inheritance01:39

Chromosomal Theory of Inheritance

59.8K
In 1866, Gregor Mendel published the results of his pea plant breeding experiments, providing evidence for predictable patterns in the inheritance of physical characteristics. The significance of his findings was not immediately recognized. In fact, the existence of genes was unknown at the time. Mendel referred to hereditary units as “factors.”
59.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell size-dependent mRNA transcription drives proteome remodeling.

Cell reports·2026
Same author

Highly Constrained Kinetic Models for Single-Cell Gene Expression Analysis.

bioRxiv : the preprint server for biology·2026
Same author

Targeting the IL-8 axis in high-risk myelodysplastic syndromes: a clinical experience with antibody blockade combined with decitabine/cedazuridine.

Leukemia & lymphoma·2026
Same author

Detection of a sequence feature for recursive splicing.

bioRxiv : the preprint server for biology·2026
Same author

TAD boundaries and gene activity are uncoupled.

eLife·2026
Same author

U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAs.

Molecular cell·2026

Related Experiment Video

Updated: Jan 26, 2026

Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling
06:37

Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling

Published on: February 9, 2010

31.9K

Structure and Function in Drosophila Chromosomes: Visualizing Topological Domains.

Daniel R Larson1

  • 1Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA.

Molecular Cell
|April 6, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel imaging method (Hi-M) to observe chromosome structure. They found that topological domains within nuclei dynamically change when gene transcription is activated.

More Related Videos

Studying Mitochondrial Structure and Function in Drosophila Ovaries
09:53

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Published on: January 4, 2017

24.8K
A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

69.7K

Related Experiment Videos

Last Updated: Jan 26, 2026

Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling
06:37

Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling

Published on: February 9, 2010

31.9K
Studying Mitochondrial Structure and Function in Drosophila Ovaries
09:53

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Published on: January 4, 2017

24.8K
A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

69.7K

Area of Science:

  • Genetics
  • Cell Biology
  • Developmental Biology

Background:

  • Understanding chromosome organization is crucial for gene regulation.
  • Dynamic changes in nuclear architecture influence cellular processes.

Purpose of the Study:

  • To develop a new sequential imaging technique for high-resolution chromosome observation.
  • To investigate the relationship between transcriptional activity and chromosome topology.

Main Methods:

  • Development of a novel sequential imaging methodology termed Hi-M.
  • Application of Hi-M to study chromosome structure in the Drosophila blastoderm.
  • Analysis of topological domain dynamics in single nuclei.

Main Results:

  • Hi-M enables detailed observation of chromosome structure.
  • Topological domains within the Drosophila blastoderm nuclei are dynamic.
  • These domains undergo significant changes in response to transcriptional activation.

Conclusions:

  • The new Hi-M technique provides unprecedented insights into chromosome dynamics.
  • Transcriptional activation directly impacts higher-order chromosome organization.
  • This study links gene expression regulation to physical changes in nuclear architecture.