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Updated: Jan 26, 2026
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Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish
Published on: April 18, 2021
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Adamts9 is necessary for ovarian development in zebrafish
Nichole Jansch Carter1, Zachary Adam Roach1, Mckenzie Marie Byrnes1
1Department of Biology, East Carolina University, Greenville 27858, NC, USA.
General and Comparative Endocrinology
|April 6, 2019
Summary
A disintegrin and metalloprotease with thrombospondin type-1 motif, member 9 (Adamts9) is crucial for gonadal development and ovulation. Zebrafish knockouts revealed severe defects in ovary and testis formation, impacting reproductive capabilities.
Area of Science:
- Developmental Biology
- Reproductive Biology
- Genetics
Background:
- The gene A disintegrin and metalloprotease with thrombospondin type-1 motif, member 9 (Adamts9) is upregulated during ovulation in vertebrates.
- Previous studies were limited by embryonic lethality in knockout models, hindering the understanding of Adamts9 function in adult reproduction.
Purpose of the Study:
- To investigate the role of Adamts9 in ovulation and gonadal development using a zebrafish knockout model.
- To characterize the phenotypic consequences of Adamts9 deficiency in adult zebrafish.
Main Methods:
- Generation of adamts9 knockout zebrafish using CRISPR/Cas9 technology.
- Phenotypic analysis of adult adamts9-/- zebrafish, including gonadal morphology and histology.
- Comparison of mutant fish with wildtype (wt) counterparts.
Main Results:
- Significantly reduced survival rate of adult adamts9-/- zebrafish compared to Mendelian predictions.
- A strong male bias was observed in the surviving mutants, with a severe underrepresentation of females.
- Mutant fish exhibited abnormal gonadal development, including underdeveloped ovaries, atypical ovarian-like structures with associated testicular tissue, and a complete absence of ovulated oocytes.
Conclusions:
- Adamts9 plays a critical role in normal gonadal development and sex determination in adult vertebrates.
- The zebrafish adamts9 knockout model provides novel insights into the function of Adamts9 in reproductive processes.
- This study highlights the importance of Adamts9 for successful ovulation and overall reproductive health.
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