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A novel pyrimidine based deep-red fluorogenic probe for detecting hydrogen peroxide in Parkinson's disease models
Xinghan Qiu1, Chenqi Xin1, Wenjing Qin1
1Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing 211800, PR China.
Talanta
|April 7, 2019
Summary
A new deep-red probe, PB1, selectively detects hydrogen peroxide (H2O2) in vivo. This probe shows potential for studying H2O2
Area of Science:
- Biomedical Engineering
- Neuroscience
- Chemical Biology
Background:
- Parkinson's disease (PD) significantly impacts patient quality of life and incurs substantial global healthcare costs.
- Abnormal hydrogen peroxide (H2O2) levels in the brain are linked to neurodegenerative diseases, including PD.
Purpose of the Study:
- To design and validate a novel deep-red fluorogenic probe (PB1) for in vivo detection of H2O2.
- To assess the probe's selectivity and responsiveness in biological systems.
Main Methods:
- Development of a novel deep-red H2O2 fluorogenic probe, PB1.
- Evaluation of PB1's selectivity against various reactive species, ions, and biothiols in aqueous solutions at physiological pH.
- Confocal fluorescence imaging to demonstrate PB1's response in cellular and Drosophila brain tissue models.
Main Results:
- PB1 exhibited high selectivity for H2O2, distinguishing it from other potential interfering substances.
- The probe demonstrated a robust response to H2O2 in both cellular environments and Drosophila brain tissue.
- Confocal imaging confirmed the probe's effectiveness for in vivo H2O2 detection.
Conclusions:
- PB1 is a highly selective and effective deep-red fluorogenic probe for detecting H2O2 in vivo.
- The probe shows significant potential as a tool for investigating the role of H2O2 in Parkinson's disease pathogenesis.