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Updated: Jan 26, 2026

Cytotoxicity Assays with Zebrafish Cell Lines
Published on: January 6, 2023
Preliminary Observation on the Cytotoxic Activity of New Chlorophyllin Derivative RCD on Human Tumour Cell Lines In
Arnaldo Pinelli1, Paolo Ferrario2, Silvio Trivulzio2
1Iraklis Galatoulas Research Unit, Department of Medical Biotechnology and Translational Medicine, University of Milan, Milan, Italy arnaldo.pinelli@unimi.it.
Background:
Chlorophyllin is used in traditional Chinese medicine because of its anticancer properties. This article describes the preparation and cytotoxic activity of a reduced chlorophyllin derivative (RCD) on tumour cell lines.
Materials And Methods:
RCD was prepared by reducing chlorophyllin with lithium aluminium hydride, and its solubility in the alkaline and organic phases are different from that of the parent compound chlorophyllin.
Results:
RCD also has different chromatographic behaviour from chlorophyllin on thin-layer chromatography and high-pressure liquid chromatography, and excitation and emission spectra. RCD has cytotoxic activity against ZR-75, MCF-7 and HT-29, human tumour cell lines. A clonogenic assay showed that the growth of tumour colonies on soft agar was reduced by RCD in a dose-dependent manner.
Conclusion:
RCD is a novel compound exhibiting anticancer activity.
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