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Updated: Jan 26, 2026

Microfluidic Device for the Separation of Non-Metastatic MCF-7 and Non-Tumor MCF-10A Breast Cancer Cells Using AC Dielectrophoresis
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Anti-MUC1 Aptamer as Carrier Tool of the Potential Radiosensitizer 1,10 Phenanthroline in MCF-7 Breast Cancer Cells

Laís N Alves1,2, Sotiris Missailidis2, Claudia A S Lage3

  • 1Laboratory of Radiobiology, Division of Medical Physics, Institute of Radioprotection and Dosimetry, Brazilian Nuclear Energy Commission, Rio de Janeiro, Brazil.

Anticancer Research
|April 7, 2019
PubMed
Abstract

Insights

Aptamers targeting Mucin 1 (aptA) can deliver the cytotoxic compound 1,10-phenanthroline to breast cancer cells. This targeted delivery shows potential for novel anticancer therapies.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cancer Research

Background:

  • Aberrantly expressed Mucin 1 glycoprotein is a key target in breast cancer.
  • Aptamers are valuable affinity agents for targeted therapeutics and drug delivery.

Purpose of the Study:

  • To investigate the feasibility of using an aptamer against Mucin 1 (aptA) to deliver 1,10-phenanthroline to MCF-7 breast cancer cells.
  • To evaluate the potential of this complex as a radiosensitizer.

Main Methods:

  • Circular dichroism spectroscopy
  • Rhodamine labeling
  • Fluorescent microscopy
  • Flow cytometry

Main Results:

  • 1,10-Phenanthroline intercalated within aptA when complexed with Fe(II) ions (Kd = 30 μM).
  • The aptA-phenanthroline complex effectively bound to and was internalized by MCF-7 breast cancer cells.

Conclusions:

  • The aptA-phenanthroline complex demonstrates specific delivery of the cytotoxic compound to cancer cells.
  • This targeted complex represents a promising strategy for anticancer therapy.
Keywords:
1,10-phenanthrolineAptamerMUC1breast cancerradiosensitizers

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