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Updated: Jan 26, 2026

Detection and Quantification of Tunneling Nanotubes Using 3D Volume View Images
Published on: August 31, 2022
Effect of tolytoxin on tunneling nanotube formation and function
Aysegul Dilsizoglu Senol1, Anna Pepe1, Clara Grudina1
1Institut Pasteur, Unité Trafic Membranaire et Pathogenèse, 25-28 Rue du Docteur Roux, 75724, Paris CEDEX 15, France.
Abstract:
Tunneling nanotubes (TNTs) are actin-containing membrane protrusions that play an essential role in long-range intercellular communication. They are involved in development of various diseases by allowing transfer of pathogens or protein aggregates as well as organelles such as mitochondria. Increase in TNT formation has been linked to many pathological conditions. Here we show that nM concentrations of tolytoxin, a cyanobacterial macrolide that targets actin by inhibition of its polymerization, significantly decrease the number of TNT-connected cells, as well as transfer of mitochondria and α-synuclein fibrils in two different cell lines of neuronal (SH-SY5Y) and epithelial (SW13) origin. As the cytoskeleton of the tested cell remain preserved, this macrolide could serve as a valuable tool for future therapies against diseases propagated by TNTs.
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