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Protein Kinase C-delta Inhibitor Peptide Formulation using Gold Nanoparticles
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Peptide generated anisotropic gold nanoparticles as efficient siRNA vectors.

Bijayananda Panigrahi1, Sourav Mishra1, Rohit Kumar Singh1

  • 1School of Biotechnology, Kalinga Institute of Industrial Technology Deemed to be University, Campus 11, Patia, Bhubaneswar, Odisha 751024, India.

International Journal of Pharmaceutics
|April 7, 2019
PubMed
Summary

New peptide-gold nanoparticles (LP-GNPs) efficiently deliver siRNA for gene silencing in colon cancer cells. These novel nanoparticles show promise as an effective RNAi therapeutic delivery system for oncotherapy.

Keywords:
GAPDHGene silencingGold nanoparticlesPeptidesiRNA

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Area of Science:

  • Nanotechnology
  • Biotechnology
  • Oncology

Background:

  • Tryptophan-containing peptides exhibit cell-penetrating properties.
  • Gold nanoparticles (GNPs) are explored for biomedical applications.
  • Efficient delivery of small interfering RNA (siRNA) is crucial for RNA interference (RNAi) therapeutics.

Purpose of the Study:

  • To design and synthesize novel peptide-gold nanoparticles (LP-GNPs) using sunlight.
  • To evaluate the efficiency of LP-GNPs in siRNA delivery and gene silencing.
  • To compare the efficacy of LP-GNPs with existing delivery systems.

Main Methods:

  • Synthesis of eight linear hexapeptides and their use in gold nanoparticle formation under sunlight.
  • Characterization of LP-GNPs using UV-Vis spectroscopy, Transmission Electron Microscopy (TEM), and Dynamic Light Scattering (DLS).
  • Assessment of siRNA binding affinity via gel electrophoresis and cellular uptake via Fluorescence Microscopy.
  • In vitro gene silencing evaluation in colon cancer cells and comparison with Lipofectamine 2000.

Main Results:

  • Peptide-generated gold nanoparticles (LP-GNPs) were successfully synthesized and characterized.
  • LP-GNPs demonstrated strong binding affinity for siRNA and significant cellular uptake.
  • LP-GNPs effectively down-regulated GAPDH gene expression in colon cancer cells.
  • Anisotropic LP7-GNPs showed comparable gene silencing efficacy to Lipofectamine 2000 in serum-containing media.

Conclusions:

  • Peptide-gold nanoparticle conjugates represent a promising platform for siRNA delivery.
  • This novel delivery system offers a new approach for RNAi therapeutics in cancer treatment.
  • LP-GNPs provide an effective and potentially improved method for oncotherapy.