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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

24.8K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
24.8K
Alternative RNA Splicing02:18

Alternative RNA Splicing

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RNA Splicing01:32

RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

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Drug Discovery: Overview01:26

Drug Discovery: Overview

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Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
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Related Experiment Video

Updated: Jan 26, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

6.4K

Alternative splicing, RNA-seq and drug discovery.

Shanrong Zhao1

  • 1Pfizer Worldwide Research and Development, Cambridge, MA 02139, USA.

Drug Discovery Today
|April 7, 2019
PubMed
Summary

Alternative splicing (AS) generates protein diversity and impacts human diseases. Advanced RNA sequencing and computational tools enable large-scale AS studies for improved diagnostics and therapeutics.

Area of Science:

  • Molecular Biology
  • Genetics
  • Bioinformatics

Background:

  • Alternative splicing (AS) is a key mechanism regulating gene expression, increasing proteome diversity.
  • Dysregulation of AS is implicated in various human diseases.
  • AS variants can influence disease progression and therapeutic outcomes.

Purpose of the Study:

  • To highlight the significance of AS in gene expression and its role in human health and disease.
  • To underscore the impact of recent technological advancements on AS research.
  • To emphasize the potential of AS studies in precision medicine.

Main Methods:

  • Utilizing RNA sequencing (RNA-seq) for transcriptome-wide AS analysis.
  • Employing computational tools for high-resolution AS studies.

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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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  • Analyzing AS variations across human populations.

    • Main Results:

    • RNA-seq and computational tools enable unprecedented scale and resolution in AS studies.
    • AS plays a critical role in disease etiology and drug resistance.
    • Splice variants serve as potential biomarkers.

    Conclusions:

    • Understanding AS variation is crucial for advancing disease diagnosis and patient care.
    • Targeting AS offers a promising avenue for novel therapeutic development.
    • AS research is fundamental to the era of precision medicine.