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Human Blastocyst Biopsy and Vitrification
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Safer Vitrification of Mouse and Human Embryos Using the Novel Cryoroom Vitrification System for Assisted
H Inui1, J Mizuno2, E Kikuchi1
1Inui Institute for Frontier Reproductive Medicine and Infertility, Inui Maternity Clinic, Koriyama, Fukushima, Japan.
Cryo Letters
|April 8, 2019
Summary
The Cryoroom vitrification device is safe and effective for assisted reproductive technology (ART), showing no difference in embryo survival or development compared to existing methods.
Area of Science:
- Reproductive biology
- Cryopreservation techniques
- Assisted Reproductive Technology (ART)
Background:
- Vitrification is a key technique in ART.
- Current methods often require specialized skills for embryo handling.
- Excess vitrification solution can be problematic.
Purpose of the Study:
- To assess the efficacy and safety of the Cryoroom vitrification device.
- To compare Cryoroom with the established Cryotop device.
Main Methods:
- Vitrification of mouse and human embryos using Cryoroom and Cryotop.
- In vitro assessment of embryo developmental potency.
- Microarray analysis of mouse blastocysts vitrified with both devices.
Main Results:
- No significant differences in survival or developmental progress between Cryoroom and Cryotop for mouse and human embryos.
- In silico analysis showed no detrimental changes in DNA methylation with Cryoroom.
- Device design may influence gene expression in vitrified embryos.
Conclusions:
- Cryoroom is a safe and effective vitrification device for ART.
- It demonstrates comparable performance to Cryotop.
- Cryoroom shows potential as a revolutionary tool in ART.
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