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Updated: Jan 26, 2026
08:32
A Small Animal Model of Ex Vivo Normothermic Liver Perfusion
Published on: June 27, 2018
14.3K
Simulation of Hypothermic Perfusion of Rabbit Livers for Cryopreservation Applications.
P B Rodríguez1, A O Fernández2, A Corral3
1Departamento de Física Aplicada III. Escuela Superior de Ingenieros, Universidad de Sevilla.
Cryo Letters
|April 8, 2019
Summary
This study simulates cryoprotectant diffusion in a rabbit liver using computational fluid-dynamics. Results show dimethyl sulfoxide (Me2SO) can evenly perfuse the organ under hypothermic conditions with constant parameters.
Area of Science:
- Cryobiology
- Biomedical Engineering
- Computational Modeling
Background:
- Precise control of cryoprotectant (CPA) perfusion is crucial for biological material cryopreservation.
- Understanding CPA concentration dynamics within tissues and organs is essential for process optimization.
Purpose of the Study:
- To simulate the diffusion and perfusion of dimethyl sulfoxide (Me2SO) and phosphate-buffered saline (PBS) within a rabbit liver.
- To investigate the feasibility of achieving uniform CPA distribution during hypothermic perfusion.
Main Methods:
- Utilized COMSOL software for computational fluid-dynamics simulations.
- Modeled hypothermic perfusion with constant temperature and pressure at the organ's entrance.
Main Results:
- Simulations demonstrated increasing Me2SO concentration within the liver's porous structure, reaching saturation.
- Perfusion speed and pressure variations within the organ remained minimal over time.
Conclusions:
- Finite element modeling confirms that uniform Me2SO loading of a rabbit liver is achievable under hypothermic conditions.
- Maintaining constant perfusion parameters ensures even cryoprotectant distribution.
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