Related Experiment Video
Updated: Jan 26, 2026
A Small Animal Model of Ex Vivo Normothermic Liver Perfusion
Published on: June 27, 2018
Simulation of Hypothermic Perfusion of Rabbit Livers for Cryopreservation Applications
P B Rodríguez1, A O Fernández2, A Corral3
1Departamento de Física Aplicada III. Escuela Superior de Ingenieros, Universidad de Sevilla.
Background:
In cryopreservation of biological material, it is very important to precise control the perfusion of cryoprotectants (CPA) inside the sample. CPA concentration inside tissues and organs during the process was measured in a few studies. The simulation of the CPA perfusion inside the organ is still necessary to understand and optimize this complex process.
Objective:
This study simulates the diffusion of Me2SO and PBS in a rabbit liver.
Materials And Methods:
The software COMSOL for computational fluid-dynamics has been used. A hypothermic perfusion is simulated where temperature and pressure at the entrance of the organ are constant.
Results:
The simulation shows that Me2SO concentration increases within the porous medium until saturation. The variation of perfusion speed and pressure inside the organ is almost null with time.
Conclusion:
Finite elements modelling shows that under hypothermic conditions it is possible a full and even loading of this organ with Me2SO, keeping constant the perfusion parameters.
Related Concept Videos
01:17Assessment of Diffusion and Perfusion
The Role of Diffusion in Respiration
Diffusion is the process by which molecules move from an area of higher concentration to an area of lower concentration. In the respiratory system, this...
01:27Liver Histology
Hepatocytes perform a variety of essential functions. They secrete...
01:30Liver Physiology
Metabolic Regulation:
The liver is the central organ involved in regulating blood composition. It stabilizes blood glucose levels, maintaining them within the range of 70–110 mg/dL. When these levels drop, the liver breaks down glycogen reserves and releases glucose into the bloodstream. It can...
01:24Liver Regeneration
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
01:17Gross Anatomy of the Liver
Located under the diaphragm, the liver is almost entirely ensconced within the rib cage, providing it with substantial protection. Except for the superior most bare area, the liver's surface is...
01:26Diseases of the Liver and Gallbladder
Cirrhosis is characterized by the scarring of hepatic lobules in the liver, which are replaced by fibrous tissue, affecting the liver's normal functioning. NAFLD, on the other hand, is caused by an excessive build-up of fat in the liver, not...