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Isochoric Containers and Its Frontier between Cryopreservation and Sterilization.
1Centro Universitario Tonalá, Universidad de Guadalajara, Tonalá Jalisco, México. abril.angulo@academicos.udg.mx.
Cryo Letters
|April 8, 2019
Summary
Isochoric containers may prevent freezing damage by keeping water liquid below 0°C. Mathematical modeling of dielectric constant (k’) suggests this state, aligning with experimental observations and explaining reduced biological harm.
Area of Science:
- Physical Chemistry
- Materials Science
Background:
- Isochoric containers are proposed for reducing freezing damage below the freezing point.
- Understanding the physical state of water in these containers is crucial for assessing their effectiveness.
Purpose of the Study:
- To mathematically determine the dielectric constant (k') of water in an isochoric container.
- To investigate the influence of nucleated volume on k' and compare with experimental data.
Main Methods:
- Modeling nucleation arrangements within a cylindrical capacitor filled with water.
- Examining changes in capacitance and dielectric constant (k') due to ice Ih formation.
Main Results:
- Dielectric constant (k') decreased proportionally with nucleated volume.
- Nucleation models did not fully reproduce experimental behavior.
- Lowest k' values were observed with nucleation over the internal electrode.
Conclusions:
- Calculated k' values suggest water remains liquid between -4°C and 0°C in isochoric containers.
- This liquid state may explain the absence of biological damage in this temperature range.
- Further research is needed to reconcile model predictions with experimental findings.
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