Folate content analysis of wheat cultivars developed in the North China Plain

Folates are essential micronutrients in the human diet. Germplasm rich in folates can be used as genetic resource for diet and breeding to produce new varieties with enhanced folates. To investigate the natural variation of folates among wheat cultivars and identify high folate materials for breeding, we studied the grain folate contents of 360 wheat samples consisting of 315 wheat genotypes grown in North China using the high performance liquid chromatography coupled with mass spectrometry (HPLC-MS/MS) method. The total folate content among wheat genotypes ranged from 10.15 ± 2.86 to 91.44 ± 5.64 µg per 100 g grains, thus showing a remarked variation. Fifty-two wheat cultivars, such as Henong58-3, were identified as good sources of folates. 5-Formyltetrahydrate and 5-methyltetrahydrate were found to be the two major folate derivatives in wheat germplasm. In addition, we found that environment factor also had significant effect on folate production. This investigation can help wheat breeders for folate improvement.