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Genotyping Mycoplasma gallisepticum by multilocus sequence typing.

Katinka Bekő1, Zsuzsa Kreizinger1, Kinga M Sulyok1

  • 1Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária körút 21, Budapest 1143, Hungary.

Veterinary Microbiology
|April 9, 2019
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Summary
This summary is machine-generated.

A new multilocus sequence typing (MLST) assay effectively distinguishes Mycoplasma gallisepticum strains, aiding in disease control for poultry. This method is crucial for epidemiological investigations and differentiating vaccine from field strains.

Keywords:
DIVAEpidemiologyGenotypingMLSTMycoplasm gallisepticum

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Area of Science:

  • Veterinary Microbiology
  • Bacterial Genomics
  • Epidemiology

Background:

  • Mycoplasma gallisepticum infection causes significant economic losses in the poultry industry due to respiratory and reproductive diseases.
  • Controlling M. gallisepticum relies on M. gallisepticum-free flocks, necessitating effective monitoring and diagnostic tools.
  • Current methods for distinguishing M. gallisepticum strains are insufficient for detailed epidemiological investigations.

Purpose of the Study:

  • To develop and validate a multilocus sequence typing (MLST) assay for Mycoplasma gallisepticum.
  • To assess the assay's ability to determine phylogenetic distances and discriminate between M. gallisepticum strains.
  • To evaluate the utility of the MLST assay for epidemiological surveillance and strain differentiation.

Main Methods:

  • Selection of six housekeeping gene loci (atpG, dnaA, fusA, rpoB, ruvB, uvrA) based on genomic location and diversity.
  • Development of an MLST assay utilizing the selected loci.
  • Genotyping of 130 M. gallisepticum strains using the developed MLST method.

Main Results:

  • The MLST assay identified 57 unique sequence types (STs) among 130 M. gallisepticum strains.
  • A high Simpson's index of diversity (0.96) indicated excellent discriminatory power of the assay.
  • The method successfully linked related strains from different farm outbreaks and differentiated vaccine from field strains.

Conclusions:

  • The developed MLST assay is a robust tool for discriminating Mycoplasma gallisepticum strains.
  • This assay is suitable for epidemiological investigations, tracking disease spread, and distinguishing between vaccine and field isolates.
  • The findings highlight the role of international trade in the dissemination of M. gallisepticum strains.