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Enteral perfusion in the pig.

R M Tolls, M A Imamovic, M P McGarry

Laboratory Animal Science

|August 1, 1986

Summary

A new surgical technique allows for colon catheterization in piglets for in vivo absorption studies. This method enables fluid administration without restraining the animals, preventing sepsis for up to two weeks.

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Area of Science:

Veterinary surgery
Gastrointestinal physiology
Animal models in research

Background:

In vivo studies of colonic absorption require reliable methods for administering solutions to the colon.
Previous techniques may involve animal restraint or pose risks of sepsis.
Piglets are a suitable model for gastrointestinal research due to physiological similarities with humans.

Purpose of the Study:

To develop and evaluate a surgical technique for chronic catheterization of the piglet colon.
To enable intermittent or continuous perfusion of the colon in unanesthetized, unrestrained piglets.
To assess the feasibility of maintaining catheter patency and preventing systemic sepsis during the study period.

Main Methods:

A simple laparotomy was performed on piglets to access the colon.
Catheters were tunneled subcutaneously to a dorsal parascapular exit site.
The technique aimed to prevent self-removal of the catheter by the animal.
Systemic sepsis was monitored to assess animal health.

Main Results:

Successful catheter insertion and tunneling were achieved in four piglets.
The animals remained free of systemic sepsis for up to two weeks post-surgery.
The surgical site allowed for intermittent or continuous colonic perfusion without physical restraint.
The pig's anatomy helped prevent catheter dislodgement.

Conclusions:

The described surgical technique provides a viable method for chronic colonic catheterization in piglets.
This approach facilitates in vivo studies of colonic absorption and perfusion in an unrestrained model.
The method minimizes the risk of sepsis and catheter removal, supporting longer-term studies.

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