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Updated: Jan 26, 2026

Preparation of Peripheral Nerve Stimulation Electrodes for Chronic Implantation in Rats
Published on: July 14, 2020
Protocol for Construction of Rat Nerve Stimulation Cuff Electrodes
Manolo U Rios1, Jesse E Bucksot2, Kimiya C Rahebi3
1School of Brain and Behavioral Sciences, The University of Texas at Dallas, Richardson, TX 75080-3021, USA; manolo.rios@utsouthwestern.edu (M.U.R.); kilgard@utdallas.edu (M.P.K.), seth.hays@utdallas.edu (S.A.H.).
Abstract:
Peripheral nerve stimulation has emerged as a platform therapy to treat a wide range of disorders. Continued development and translation of these strategies requires that researchers have access to reliable, customizable electrodes for nerve stimulation. Here, we detail procedures to build three different configurations of cuff electrodes with varying numbers and orientations of contacts for nerve stimulation in rats. These designs are built with simple, widely available materials, using platinum-iridium electrodes assembled into polyurethane tubing. Moreover, the designs can easily be customized to increase versatility and individualize for specific stimulation applications. This protocol provides a resource to facilitate the construction and customization of stimulation cuffs to support preclinical nerve stimulation research.
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