Restraint stress in biomedical research: a review

The use of restraint or immobilization for investigations of animal physiology, pathology and pharmacology has an extensive history. The major use of this technique has been as a "stressor" for the induction of stress response syndromes in animals. Many such syndromes have been characterized from the behavioral level to the neurochemical concomitants of stress. As a consequence of this particular use of the restraint procedure, much information concerning drug effects on stress response syndromes has been obtained. Indeed, many researchers in the area of gastrointestinal drugs routinely screen their new compounds in a restraint model of gastric stress ulcer. The purpose of this review is to present for researchers, a summary of the methods for, the parameters of, and known drug effects on, restraint-induced pathology. In our experience, this technique has proven to be a very useful one for the examination of both central and peripheral mechanisms of stress-related disorders, as well as for studying drug effects upon these disorders.