Production and Characterization of Antibody Against 3'-OH-T-2 Toxin

Antibody raised against T-2 toxin cross-reacted poorly with 3'-OH-T-2 toxin. A new immunogen was prepared by conjugation of hemisuccinate (HS) of 3'-OH-T-2 toxin to bovine serum albumin (BSA). Antibodies against 3'-OH-T-2 toxin were demonstrated by a radioimmunoassay 10 wk after immunization of rabbits with this new immunogen using tritiated 3'-OH-T-2 toxin as the testing ligand. Highest titers (1:6,000) were obtained 17 wk after immunization and two booster injections. The antibodies had good cross-reactivity with T-2 toxin, acetyl-T-2 toxin and 3'-OH-acetyl-T-2 toxin. The relative cross-reactivity of this antibody with 3'-OH-T-2, acetyl-T-2, T-2, 3'-OHacetyl-T-2, 3'-OH-T-2-HS, T-2 isomer, HT-2 and 3'-OH-HT-2 was 1, 3, 4, 5, 15, 30, 45 and 175, respectively. No crossreaction was found when 3'-OH-T-2 triol, T-2-triol, T-2-tetraol, DAS and DON at a concentration of 1 μg per assay was tested. The detection limit for 3'-OH-T-2 toxin by the RIA was about 0.1 ng per assay.