Related Experiment Video
Updated: Jan 26, 2026

Bioluminescent Bacterial Imaging In Vivo
Published on: November 4, 2012
Adenosine Triphosphate Bioluminescent Assay to Enumerate Bacterial Numbers on Fresh Fish
Donn R Ward1, Kathleen A LaROCCO1, Debra J Hopson1
1Seafood Processing Research Laboratory, Virginia Polytechnic Institute, Hampton, Virginia 23669 and United Technologies, Packard, Downers Grove, Illinois 60515.
Abstract:
The microbial quality of fresh fish was determined by using a bacterial ATP bioluminescent assay. Assay procedures included differential filtration followed by enzymatic degradation of somatic ATP. This technique limited interference from non-bacterial ATP sources and thus bacterial ATP was quantitated by using the luciferin-luciferase reaction in an automated luminometer. Good correlation (r = .96) was obtained from four species of finfish when microbial counts, determined from ATP analyses, were compared to counts determined by using conventional plate count procedures.
Related Concept Videos
Osmoregulation in Fishes
Bacterial Signaling
Segregation in Fresh Concrete
Bleeding in Fresh Concrete
Bleeding can cause several issues in the concrete structure. Sometimes, the rising water gets trapped beneath large aggregate...
Bacterial Transformation
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
FISH - Fluorescent In-situ Hybridization

