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HPLC Analysis of Oxytetracycline Residues in Honey
Peter Sporns1, Suet Kwan1, Lawrence A Roth1
1Department of Food Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5 and Food laboratory Services Branch, Alberta Agriculture, O.S. Longman Building, Edmonton, Alberta, Canada T6H 4P2.
Abstract:
Oxytetracycline (OTC), also known commercially as Terramycin, was determined to be more stable in honey than in buffered aqueous solutions at similar pH values and temperatures. A rapid high performance liquid chromatography (HPLC) method was developed to detect and quantitate OTC using a 1:1 dilution (wt/wt) of honey samples in water. Using 355 nm as the wavelength of detection, amounts as low as 0.5 μg/ml could be detected in the above solution. The limits of detection were lowered considerably by a double extraction procedure.
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