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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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Atomic Orbitals02:44

Atomic Orbitals

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An atomic orbital represents the three-dimensional regions in an atom where an electron has the highest probability to reside. The radial distribution function indicates the total probability of finding an electron within the thin shell at a distance r from the nucleus. The atomic orbitals have distinct shapes which are determined by l, the angular momentum quantum number. The orbitals are often drawn with a boundary surface, enclosing densest regions of the cloud.
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Intermolecular Forces03:13

Intermolecular Forces

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Atoms and molecules interact through bonds (or forces): intramolecular and intermolecular. The forces are electrostatic as they arise from interactions (attractive or repulsive) between charged species (permanent, partial, or temporary charges) and exist with varying strengths between ions, polar, nonpolar, and neutral molecules. The different types of intermolecular forces are ion–dipole, dipole–dipole, hydrogen bonds, and dispersion; among these, dipole–dipole, hydrogen...
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The Energies of Atomic Orbitals03:21

The Energies of Atomic Orbitals

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In an atom, the negatively charged electrons are attracted to the positively charged nucleus. In a multielectron atom, electron-electron repulsions are also observed. The attractive and repulsive forces are dependent on the distance between the particles, as well as the sign and magnitude of the charges on the individual particles. When the charges on the particles are opposite, they attract each other. If both particles have the same charge, they repel each other.
30.0K
Intermolecular vs Intramolecular Forces03:00

Intermolecular vs Intramolecular Forces

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Intermolecular forces (IMF) are electrostatic attractions arising from charge-charge interactions between molecules. The strength of the intermolecular force is influenced by the distance of separation between molecules. The forces significantly affect the interactions in solids and liquids, where the molecules are close together. In gases, IMFs become important only under high-pressure conditions (due to the proximity of gas molecules). Intermolecular forces dictate the physical properties of...
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Atomic Structure01:33

Atomic Structure

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Bacterial Immobilization for Imaging by Atomic Force Microscopy
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Noninvasive Subcellular Imaging Using Atomic Force Acoustic Microscopy (AFAM).

Xiaoqing Li1, Ang Lu2, Wenjie Deng3

  • 1Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. d201677426@hust.edu.cn.

Cells
|April 10, 2019
PubMed
Summary
This summary is machine-generated.

Atomic Force Acoustic Microscopy (AFAM) offers a new way to see inside cells without damage. This technique provides high-resolution nanoscale images of subcellular structures, advancing cell biology research.

Keywords:
atomic force acoustic microscopyimage fusion algorithmparameters optimizationsubcellular structural image atlas

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Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Nanotechnology

Background:

  • Conventional imaging techniques struggle to visualize subcellular structures with high resolution non-destructively.
  • Understanding internal cell architecture is crucial for diverse biological fields.

Purpose of the Study:

  • To develop and optimize an Atomic Force Acoustic Microscopy (AFAM) approach for high-resolution, non-destructive imaging of intracellular structures.
  • To enhance the visualization of nanoscale subcellular features.

Main Methods:

  • Optimization of AFAM imaging parameters: scanning speeds, feedback configurations, and acoustic frequencies.
  • Combination of acoustic amplitude and phase signals for improved image contrast.
  • Generation of pseudo-color images to highlight subcellular features.

Main Results:

  • Achieved high spatial resolution imaging of subcellular features, including pseudopodia, membranes, and nucleus-like structures.
  • Generated a nanoscale subcellular structural image atlas with clearer visualization compared to conventional methods.
  • Demonstrated the stabilization of morphological signals and increased acoustic signal amplitude.

Conclusions:

  • Transmission AFAM is a powerful tool for non-destructive cell imaging at the nanoscale.
  • This technique provides a clearer understanding of subcellular structures, aiding diverse biological research.
  • The study lays a foundation for advancing cell structure analysis and biological evolution studies.