Characterization of Lignin Extracted from Willow by Deep Eutectic Solvent Treatments

Purity, morphology, and structural characterization of synthesized deep eutectic solvent (DES)-lignins (D_6h, D_9h, D_12h, D_18h, D_24h) extracted from willow (Salix matsudana cv. Zhuliu) after treatment with a 1:10 molar ratio of choline chloride and lactic acid at 120 °C for 6, 9, 12, 18, and 24 h were carried out. The purity of DES-lignin was ~95.4%. The proportion of hydrogen (H) in DES-lignin samples increased from 4.22% to 6.90% with lignin extraction time. The DES-lignin samples had low number/weight average molecular weights (1348.1/1806.7 to 920.2/1042.5 g/mol, from D_6h to D_24h) and low particle sizes (702⁻400 nm). Atomic force microscopy (AFM) analysis demonstrated that DES-lignin nanoparticles had smooth surfaces and diameters of 200⁻420 nm. Syringyl (S) units were dominant, and total phenolic hydroxyl content and total hydroxyl content reached their highest values of 2.05 and 3.42 mmol·g^-1 in D_12h and D_6h, respectively. β-Aryl ether (β-O-4) linkages were eliminated during DES treatment.