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Updated: Jan 26, 2026

Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice
Published on: May 16, 2021
Preparation of Microparticles Capable of Glucose-Induced Insulin Release under Physiological Conditions
Kentaro Yoshida1, Kazuma Awaji2, Seira Shimizu3
1School of Pharmaceutical Sciences, Ohu University 31-1 Misumido, Tomita-Machi, Koriyama, Fukushima 963-8611, Japan. k-yoshida@pha.ohu-u.ac.jp.
Abstract:
Hydrogen peroxide (H₂O₂)-sensitive layer-by-layer films were prepared based on combining phenyl boronic acid (PBA)-modified poly(allylamine) (PAH) with shikimic acid (SA)-modified-PAH through boronate ester bonds. These PBA-PAH/SA-PAH multilayer films could be prepared in aqueous solutions at pH 7.4 and 9.0 in the presence of NaCl. It is believed that the electrostatic repulsion between the SA-PAH and PBA-PAH was diminished and the formation of ester bonds between the SA and PBA was promoted in the presence of NaCl. These films readily decomposed in the presence of H₂O₂ because the boronate ester bonds were cleaved by an oxidation reaction. In addition, SA-PAH/PBA-PAH multilayer films combined with glucose oxidase (GOx) were decomposed in the presence of glucose because GOx catalyzes the oxidation of D-glucose to generate H₂O₂. The surfaces of CaCO₃ microparticles were coated with PAH/GOx/(SA-PAH/PBA-PAH)₅ films that absorbed insulin. A 1 mg quantity of these particles released up to 10 μg insulin in the presence 10 mM glucose under physiological conditions.
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