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Updated: Jan 26, 2026

Inducible and Reversible Dominant-negative DN Protein Inhibition
Published on: January 7, 2019
High-throughput identification of dominant negative polypeptides in yeast
Michael W Dorrity1, Christine Queitsch1, Stanley Fields2,3
1Department of Genome Sciences, University of Washington, Seattle, WA, USA.
Abstract:
Dominant negative polypeptides can inhibit protein function by binding to a wild-type subunit or by titrating a ligand. Here we use high-throughput sequencing of libraries composed of fragments of yeast genes to identify polypeptides that act in a dominant negative manner, in that they are depleted during cell growth. The method can uncover numerous inhibitory polypeptides for a protein and thereby define small inhibitory regions, even pinpointing individual residues with critical functional roles.
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