An Improved Cryopreservation Protocol for Mentha spp. Based on Pvs3 as the Cryoprotectant

Area of Science:

• Plant science
• Cryobiology
• Genetic resource conservation

Background:

• Vitrification is a key technique for cryopreserving Mentha spp. genetic resources.
• Effective cryopreservation is crucial for conserving valuable mint germplasm.

Purpose of the Study:

• To compare the cryopreservation response of 20 Mentha species and hybrids.
• To evaluate the efficacy of two cryoprotectants, PVS2 and PVS3.

Main Methods:

• Cryopreservation of 153 Mentha spp. accessions using in vitro plants.
• Application of Plant Vitrification Solution 2 (PVS2) and PVS3 as cryoprotectants.

Main Results:

• PVS2 was effective for most species, with exceptions noted for M. requienii and M. villosanervata.
• PVS3 significantly increased explant regrowth post-rewarming.
• PVS3 application resulted in less frequent and less severe endophyte outbreaks compared to PVS2.

Conclusions:

• Both PVS2 and PVS3 are viable cryoprotectants for Mentha spp.
• PVS3 is preferred due to higher regeneration success and reduced endophyte risk.
• PVS3 should be the preferred cryoprotectant for future Mentha spp. germplasm preservation.