Related Experiment Video
Updated: Jan 26, 2026

Bottlenose Dolphin Tursiops truncatus Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization
Published on: August 21, 2017
The Assessment of Cryopreservation on the Quality of Endangered Oravka Rooster Spermatozoa Using Casa and Cytometry
A Svoradová1, L Kuželová2, J Vašíček3
1Constantine the Philosopher University in Nitra, Faculty of Natural Sciences, Nitra. svoradovaandreal@gmail.com.
Background:
Preservation of genetic resources in gene bank is necessary for conservation of endangered poultry species.
Objective:
This study is to characterize Oravka rooster semen quality.
Materials And Methods:
Heterospermic pool was diluted (1:1 by volume) in a freeze medium composed of a commercial diluent (Kobidil+, K), 8% dimethyl sulphoxide (DMSO) or 8% ethylene glycol (EG) or 8% glycerol (GL), and then frozen in liquid nitrogen vapour.
Results:
Spermatozoa in the GL/K+ had significantly higher number of motile and progressively moving spermatozoa (p < 0.05) than in DMSO/K+ and EG/K+ groups. The percentage of apoptotic and necrotic spermatozoa were significantly higher in the DMSO/K+ and EG/K+ groups compared with the GL/K+ group. Based on the total motility, progressive movement parameters and viability, our study showed that 8% GL diluted in Kobidil+ provided the highest cryoprotective effect on the Oravka rooster spermatozoa.
Related Concept Videos
Quality of Water
Quality Control
Quality control helps track data, visualize trends, and identify variations, making it easier to detect deviations that may affect the accuracy of an analysis. One way to do this is by generating a quality control chart, which...
Quality Assurance
Pulse amplitude and quality
A weak or absent pulse may indicate reduced cardiac output or poor left ventricular contraction, which can be signs of cardiovascular dysfunction or...
Testing Water Quality
Flow Cytometry
In...

