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Field Performance of Cryopreserved Seed-derived Maize Plants
M Arguedas1, A Villalobos1, D Gómez1
1Laboratory for Plant Breeding and Conservation of Genetic Resources, Centro de Bioplantas, Universidad de Ciego de Ávila, Ciego de Ávila 69450, Cuba.
Background:
Cryo-preservation of plant materials in liquid nitrogen (LN) has been described as a suitable technology to conserve genetic resources of several species. However, the potential effects of LN in the subsequent plant growth in the field should be studied before large-scale implementation of cryopreserved germplasm banks.
Objective:
To describe the field performance of cryopreserved seed-derived maize adult plants.
Materials And Methods:
Germination percentage and numbers of leaves and ears per plant, internodes in stems, middle - aged leaf length, plant height, ear traits and weight of 100 seeds were recorded.
Results:
Statistically significant differences between adult plants derived from cryopreserved seeds and the control treatment were not observed (t-test, p=0.05).
Conclusion:
The results presented confirm at the phenotype level the effectiveness of maize seed cryostorage to preserve and regenerate true-to-type plants.
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