Protective Effect of Vitrified-warmed Media with Clove Bud (Syzygium aromaticum) Extract on Mouse Oocytes and
S Bahmanpour1, A Bakhtari2, B Abouhamzeh3
1Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran. Department of Reproductive Biology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
Background:
Although oocyte vitrification has become an essential part of infertility treatments, the formation of reactive oxygen species can reduce the quality of oocytes.
Objective:
Protective effects of vitamin E or clove bud extract in the vitrification and warming media of vitrified mature oocytes were evaluated on blastocysts derived from the warmed oocytes.
Materials And Methods:
Oocytes were vitrified-warmed with vitamin E (0, 50, 100, 200 and 400 mM) or hydroethanolic extract of clove bud (0, 5, 10, 20 and 40 µg/ml) in vitrification and warming media, and resultant blastocysts were evaluated.
Results:
Mid concentrations of these antioxidants (10 and 20 µg/ml of clove bud extract and 100 and 200 µM of vitamin E) improved the blastocyst formation (P<0.05). The expression of catalase (P<0.01) and superoxide dismutase-1 (Sod1) genes, as well as the inner cell mass number (P<0.05), were significantly less in the blastocyst of the control (untreated) vitrified oocytes, however these levels were restored to normal by clove bud extract. In both antioxidant groups Bcl2l1 gene expression was promoted in comparison to the controls (P<0.05).
Conclusion:
Clove bud extract, with antioxidant and anti-apoptotic properties, may serve to prevent or reduce oxidative stress condition of oocyte vitrification.
Related Concept Videos
Resultant of a General Distributed Loading
Examples such as load distribution due to wind and load distribution on a bridge illustrate how this concept is used to analyze and design safe, reliable structures under variable loading conditions. Most structures, such as residential buildings, bridges, and towers, are...
Resultant Moment: Scalar Formulation
To determine the resultant moment, the moments caused by all the forces in a system in the x-y plane are considered. Positive moments are typically...
Resultant Moment: Vector Formulation
The resultant moment of a system of forces can be calculated through vector formulation. For example, if we consider...
The Tongue and Taste Buds
Taste Buds and Receptors
Zones of Protection
Protective zones are defined by closed dashed lines, containing one or more components. A key characteristic of these zones is the strategic placement of...


