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Cryopreservation of Winter-dormant Apple Buds IV: Critical Temperature Variation that Can Compromise Survival
C Vogiatzi1, B Ww Grout2, A Wetten3
1Department of Plant & Environmental Sciences, University of Copenhagen, Højbakkegård Allé 13, DK-2630 Taastrup, Denmark.
Background:
Precise temperature control in several key areas during cryopreservation of dormant, winter apple buds is critical for maximal survival.
Objective:
To consider the effects of pre-harvest temperature, the duration of incubation at -30°C and variation in rewarming rate on survival.
Materials And Methods:
Dormant winter buds of Malus x domestica cultivars were harvested with two different acclimation histories and cryopreserved with variation in incubation time at -30°C. Recovery from LN using slow, intermediate and rapid rewarming was investigated as well as preservation after prolonged storage at -4°C.
Results:
The effects on survival of preharvest temperature regime and an altered -30°C incubation regime are cultivar dependent and an increase in rewarming rate has a strong negative effect on recovery.
Conclusion:
Post-thaw survival of the winter-dormant buds can be compromised by increased temperature over a period as short as 5 days prior to bud harvest. Varying incubation times at -30°C produce variable, cultivar dependent, survival and moderate increases in rewarming rates can also radically reduce survival.
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