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A Simple Fluorescent Probe for Sensing pH and its Application in E. coli Cells.

Jianbin Chao1, Xiaolu Wang2,3, Yongbin Zhang4

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|April 10, 2019
PubMed
Summary

A novel fluorescent probe detects strong acids and bases with high sensitivity and selectivity. This chemical sensor was successfully used for imaging pH changes within E. coli cells.

Keywords:
Bio-imagingExtremely acid and alkalineFluorescent probepH-sensitive

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Area of Science:

  • Analytical Chemistry
  • Chemical Sensing
  • Biophysical Chemistry

Background:

  • Accurate pH monitoring is crucial in various scientific disciplines.
  • Development of selective and sensitive fluorescent probes is essential for real-time pH measurements.
  • Existing probes may lack the required selectivity or sensitivity for specific applications.

Purpose of the Study:

  • To design and synthesize a simple fluorescent probe for detecting strong acidic and alkaline conditions.
  • To evaluate the probe's quantitative detection capabilities and selectivity.
  • To investigate the sensing mechanism and its application in biological systems.

Main Methods:

  • Synthesis of a novel fluorescent probe: (E)-2-(2-(3,4,5-Trimethoxybenzylidene)hydrazinyl) benzothiazole.
  • Spectroscopic analysis (fluorescence) to determine pH response.
  • Quantitative analysis of acid and base detection ranges and pKa values.
  • Mechanism studies involving H+ binding and deprotonation.
  • Application of the probe for pH imaging in E. coli cells.

Main Results:

  • The probe successfully detected strong acidic pH in the range of 2.60-3.53 (pKa = 2.78).
  • The probe exhibited a linear response to alkaline pH values from 9.98-10.95 (pKa = 9.32).
  • High selectivity and sensitivity were observed across the tested pH ranges.
  • Mechanism elucidated involves protonation/deprotonation of nitrogen atoms in the benzothiazole and hydrazine moieties.
  • Successful imaging of strongly acidic and alkaline environments within E. coli cells was achieved.

Conclusions:

  • The synthesized fluorescent probe offers a simple, selective, and sensitive method for detecting strong acidic and alkaline pH.
  • The probe's ability to function in a DMSO/water system and its successful application in cellular imaging highlight its potential utility.
  • This probe represents a valuable tool for quantitative pH monitoring and biological imaging applications.