Two new alkaloids from Crinum asiaticum var. japonicum

A new crinine-type alkaloid crijaponine A (1), a new galanthamine-type alkaloid crijaponine B (2), and 11 known alkaloids-ungeremine (3), lycorine (4), 2-O-acetyllycorine (5), 1, 2-O-diacetyllycorine (6), (-)-crinine (7), 11-hydroxyvittatine (8), hamayne (9), (+)-epibuphanisine (10), crinamine (11), yemenine A (12), and epinorgalanthamine (13)-were isolated from the rhizome and fruits of Crinum asiaticum var. japonicum. The structural elucidation of the isolated compounds was performed by spectroscopic methods including 2D NMR. The isolated compounds were evaluated for cytotoxicity against HeLa and HL-60 cells lines and were tested for acetylcholinesterase inhibition activity.