Receptors for advanced glycation end products is associated with autophagy in the clear cell renal cell carcinoma

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Yong Guo1, Hai-Cong Zhang2, Sheng Xue3

  • 1Department of Urology, Shanghai Tenth People's Hospital of Nanjing Medical University, Nanjing; Transplantation Centre, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Journal of Cancer Research and Therapeutics
|April 10, 2019
PubMed
Abstract

Insights

The receptor for advanced glycation end-product (RAGE) and high-mobility group box 1 (HMGB1) pathway is elevated in clear cell renal cell carcinoma (ccRCC). This pathway regulates autophagy, suggesting RAGE/HMGB1 as a potential therapeutic target for ccRCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cellular Signaling

Background:

  • The receptor for advanced glycation end-product (RAGE) is a signal transduction receptor involved in various human diseases, including cancer.
  • RAGE, in conjunction with high-mobility group box 1 (HMGB1), has been shown to regulate autophagy in carcinomas.
  • The specific role of the RAGE/HMGB1 pathway in clear cell renal cell carcinoma (ccRCC) autophagy was previously unknown.

Purpose of the Study:

  • To investigate the functional role of the RAGE/HMGB1 pathway in the regulation of autophagy in clear cell renal cell carcinoma (ccRCC).
  • To determine the expression levels of RAGE/HMGB1 and key autophagic proteins in ccRCC tissues and cell lines.

Main Methods:

  • Western blot analysis to quantify protein expression.
  • Quantitative real-time polymerase chain reaction (qRT-PCR) to assess gene expression.
  • Immunofluorescence assays to visualize protein localization and expression.

Main Results:

  • Significantly higher levels of RAGE/HMGB1 and autophagic proteins (LC3, Beclin-1, PI3K) were observed in ccRCC samples compared to adjacent normal tissues.
  • RAGE/HMGB1 was found to regulate the expression of autophagic proteins in human RCC cell lines.

Conclusions:

  • RAGE and autophagy play critical roles in the development and progression of ccRCC.
  • The RAGE/HMGB1 pathway represents a potential novel therapeutic target for ccRCC treatment.
Keywords:
Autophagyclear cell renal cell carcinomahigh-mobility group box 1receptor for advanced glycation end-product

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