Related Experiment Video
Updated: Jan 26, 2026

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
Published on: November 8, 2011
Chromatin remodeling factor lymphoid-specific helicase links with Epstein-Barr virus associated the follicular
Chunhui Ouyang1, Zhenghao Deng2, Jianhua Zhou2
1Department of Gastroenterology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
Background:
We assessed the frequency of epigenetic lesions, including lymphoid-specific helicase (LSH), 5-hydroxymethylcytosine (5-hmC) and E2F1, and the possible correlations among molecular findings, phenotype, clinical features, and outcome.
Methods:
We investigated 181 paraffin-embedded B-cell lymphoma samples using immunohistochemistry and in situ hybridization.
Results:
The levels of Ki67, LSH, 5-hmC, and E2F1 were all increased in germinal center B-cell lymphomas when compared with those in normal lymph nodes, and LSH was highly expressed in diffuse large B-cell lymphomas (DLBCLs) and Burkitt lymphomas (BLs) that were positive for Epstein-Barr virus (EBV) infection, indicating that LSH is linked to EBV infection in DLBCL and BL. Interestingly, LSH was mainly localized in the germinal centers of lymph nodes whereas 5-hmC staining localized to areas surrounding the germinal centers.
Conclusions:
These findings indicate a critical role for LSH as a biomarker and therapeutic target in follicular germinal center B-cell lymphoma.
Related Concept Videos
DNA Helicases
Nucleosome Remodeling
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Other Factors Affecting Respiration Centers
However, the ability to hold one's breath voluntarily is not limitless. When the CO2 concentration in the blood reaches a critical...
Chromatin Structure Regulates pre-mRNA Processing
The chromatin structure, especially...
Chemical Factors Affecting Respiration Centers
CO2 has a potent influence on respiration and is strictly regulated....
Transcription Elongation Factors
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...

