Related Experiment Video

Updated: Jan 26, 2026

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway
07:15

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

Published on: August 23, 2024

882

Effect of miR-29b on rats with gestational diabetes mellitus by targeting PI3K/Akt signal

H-Y Zong1, E-L Wang, Y-M Han

  • 1Department of Obstetrics and Gynecology, Juancheng People's Hospital, Heze, China. zjoo81@163.com.

European Review for Medical and Pharmacological Sciences
|April 10, 2019
PubMed
Abstract

Insights

Micro-ribonucleic acid-29b (miR-29b) plays a role in gestational diabetes mellitus (GDM) by regulating the PI3K/Akt pathway. Restoring miR-29b levels in GDM rats reduces oxidative stress and blood glucose.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Biochemistry

Background:

  • Gestational diabetes mellitus (GDM) is a significant pregnancy complication.
  • The phosphatidylinositol 3-kinase (PI3K)/serine/threonine kinase (Akt) signaling pathway is implicated in metabolic regulation.
  • Micro-ribonucleic acids (miRNAs) are emerging as key regulators in various physiological and pathological processes.

Purpose of the Study:

  • To investigate the role of micro-ribonucleic acid-29b (miR-29b) in a rat model of gestational diabetes mellitus (GDM).
  • To elucidate the mechanism involving the PI3K/Akt signaling pathway in GDM pathogenesis.
  • To assess the therapeutic potential of modulating miR-29b in GDM.

Main Methods:

  • Establishment of rat models for GDM.
  • Quantification of miR-29b, PI3K, and Akt expression using RT-PCR and Western blotting.
  • In vivo manipulation of miR-29b levels using mimics and inhibitors.
  • Assessment of oxidative stress markers (SOD, CAT, MDA) and fasting blood glucose levels.

Main Results:

  • GDM rats exhibited decreased miR-29b expression and increased PI3K/Akt signaling.
  • A significant negative correlation was observed between miR-29b and PI3K expression.
  • Administration of miR-29b mimics reduced PI3K/Akt phosphorylation, alleviated oxidative stress, and lowered blood glucose levels in GDM rats.

Conclusions:

  • MiR-29b plays a crucial role in the pathogenesis of GDM.
  • Inhibition of the PI3K/Akt signaling pathway by miR-29b contributes to its glucose-lowering and anti-oxidative effects.
  • Modulating miR-29b represents a potential therapeutic strategy for GDM.

More Related Videos

A Zebrafish Model of Diabetes Mellitus and Metabolic Memory10:03

A Zebrafish Model of Diabetes Mellitus and Metabolic Memory

Published on: February 28, 2013

26.5K
Tear-Derived Exosomal miR-15a as New Diagnostic Tool for Diabetic Retinopathy07:45

Tear-Derived Exosomal miR-15a as New Diagnostic Tool for Diabetic Retinopathy

Published on: December 30, 2025

331

Related Concept Videos

Diabetes Mellitus: Type 2 and Gestational01:22

Diabetes Mellitus: Type 2 and Gestational

Type 2 diabetes, characterized by insulin resistance, arises when the insulin receptors on cells lose responsiveness to insulin, diminishing the cell's capacity to take up glucose, resulting in elevated blood glucose levels. To receive a diagnosis of Type 2 diabetes, a series of blood glucose tests are necessary to assess whether the blood glucose falls within normal parameters. If the result is out of the normal range, a patient may be diagnosed as prediabetic or diabetic, depending on the...
4.4K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.4K
Diabetes Mellitus: Overview and Type I Subtype01:22

Diabetes Mellitus: Overview and Type I Subtype

Diabetes mellitus is a chronic metabolic disorder characterized by high blood glucose levels due to inadequate insulin production, insulin resistance, or both. The condition affects millions worldwide and can significantly impact their health and quality of life.
Type 1 diabetes is an autoimmune disease in which the immune system mistakenly attacks and destroys the insulin-producing beta cells in the pancreas. As a result, the body is unable to produce sufficient insulin, and individuals with...
5.0K
Pathophysiology of Diabetes01:20

Pathophysiology of Diabetes

Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia. The four categories of diabetes are type 1 diabetes, type 2 diabetes, other specific types of diabetes, and gestational diabetes.
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility,...
3.3K
What is Cell Signaling?02:03

What is Cell Signaling?

Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate to respond to the environment.
130.0K
Endocrine Signaling01:45

Endocrine Signaling

Endocrine cells produce hormones to communicate with remote target cells found in other organs. The hormone reaches these distant areas using the circulatory system. This exposes the whole organism to the hormone but only those cells expressing hormone receptors or target cells are affected. Thus, endocrine signaling induces slow responses from its target cells but these effects also last longer.
68.0K