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MiR-335 suppresses cell proliferation and migration by upregulating CRKL in bladder cancer

X-K Liu1, D Chen, X Li

  • 1Department of Urology, Wuhan Asia General Hospital, Wuhan, China. drxinli@yahoo.com.

European Review for Medical and Pharmacological Sciences
|April 10, 2019
PubMed
Abstract

Insights

MicroRNA-335 (miR-335) is downregulated in bladder cancer (BC) and suppresses tumor growth. Upregulating miR-335 inhibits BC cell proliferation and migration by targeting CRKL (CT10 regulator of kinase-like protein).

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Aberrant microRNA (miRNA) expression is implicated in bladder cancer (BC) pathogenesis.
  • Investigating the specific role of miR-335 in BC development is crucial for understanding disease mechanisms.

Purpose of the Study:

  • To elucidate the function of miR-335 in bladder cancer.
  • To determine the relationship between miR-335 and its potential target, CRKL, in BC.

Main Methods:

  • Real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot were used to assess miR-335 and CRKL expression.
  • Cell viability was evaluated using MTT assays.
  • Cell migration and invasion were assessed via Transwell assays.
  • Luciferase reporter assays confirmed the direct targeting of CRKL by miR-335.

Main Results:

  • miR-335 expression was significantly decreased, while CRKL expression was elevated in BC tissues compared to normal tissues.
  • CRKL was identified as a direct target of miR-335, with a negative correlation between their expression levels in BC.
  • Inhibition of CRKL or upregulation of miR-335 suppressed BC cell proliferation and migration.
  • Overexpression of CRKL could reverse the inhibitory effects of miR-335 on BC cell behavior.

Conclusions:

  • miR-335 acts as a tumor suppressor in bladder cancer.
  • miR-335 inhibits BC cell proliferation and migration, potentially through the downregulation of its target gene, CRKL.

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