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MicroRNA-345 suppresses cell invasion and migration in non-small cell lung cancer by directly targeting YAP1

M-Y Zhang1, J Lin, Y-C Kui

  • 1Department of Thoracic Surgery, the Affiliated Hospital of Weifang Medical University, Weifang, China. kuiyuchuanxh@outlook.com.

European Review for Medical and Pharmacological Sciences
|April 10, 2019
PubMed
Abstract

Insights

MicroRNA-345 (miR-345) is downregulated in non-small cell lung cancer (NSCLC). Overexpressing miR-345 inhibits NSCLC cell migration and invasion by targeting YAP1, suggesting a potential therapeutic biomarker.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Non-small cell lung cancer (NSCLC) is a leading cause of cancer-related mortality worldwide.
  • Understanding the molecular mechanisms regulating NSCLC progression, particularly cell migration and invasion, is crucial for developing effective treatments.

Purpose of the Study:

  • To elucidate the role of microRNA-345 (miR-345) in non-small cell lung cancer (NSCLC) cell migration and invasion.
  • To investigate the underlying molecular mechanism, specifically identifying the target gene regulated by miR-345.

Main Methods:

  • Analysis of miR-345 expression in 52 NSCLC tissues and matched normal lung tissues.
  • Assessment of NSCLC cell migration and invasion using transwell assays upon miR-345 manipulation.
  • Quantification of yes-associated protein 1 (YAP1) expression via Western blot and qRT-PCR.

Main Results:

  • MiR-345 was significantly downregulated in NSCLC tissues compared to normal lung tissues.
  • Overexpression of miR-345 markedly inhibited NSCLC cell migration and invasion.
  • Yes-associated protein 1 (YAP1) was identified as a direct functional target of miR-345, with inverse correlation in expression levels within NSCLC samples.

Conclusions:

  • MiR-345 suppresses NSCLC cell migration and invasion by directly targeting YAP1.
  • The miR-345/YAP1 axis represents a potential therapeutic target and biomarker for NSCLC treatment.

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