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Updated: Jan 26, 2026
Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
Published on: May 2, 2017
MiR-15b regulates cell differentiation and survival by targeting CCNE1 in APL cell lines
Zhen Yuan1, Liang Zhong2, Dongdong Liu1
1Central Laboratory of Yong-Chuan Hospital, Chongqing Medical University, Chongqing 402160, China; Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
Abstract:
MicroRNAs have been shown to be involved in various cell processes, including proliferation, apoptosis and differentiation. However, little is known about their function in granulopoiesis. In the present study, overexpression and knockdown experiments revealed that miR-15b was required to block the proliferation of NB4 and HL60 cells and induce them differentiated to granulocyte lineage. Moreover, we identified CCNE1 as a direct target of miR-15b, and demonstrated that CCNE1 was involved in cell differentiation and proliferation in acute promyelocytic leukemia cells. In addition, we demonstrated a novel pathway in which miR-15b regulated cells arrested in the G0/G1 phase and promoted terminal differentiation of cells by targeting CCNE1, which could modulate the cell cycle effort pRb in APL cells. These events blocked cell proliferation and promoted granulocyte differentiation. In conclusion, our data highlighted, for the first time, the important role of miR-15b in myeloid differentiation and suggested the potential role of miR-15b in cancer therapy.
Insights
MicroRNA-15b (miR-15b) blocks cancer cell proliferation and promotes granulocyte differentiation by targeting CCNE1. This study highlights miR-15b
Area of Science:
- Molecular Biology
- Cell Biology
- Hematology
Background:
- MicroRNAs regulate crucial cellular processes like proliferation and differentiation.
- The specific role of microRNAs in granulopoiesis remains largely unexplored.
- Acute promyelocytic leukemia (APL) is characterized by aberrant cell proliferation and differentiation.
Purpose of the Study:
- To investigate the function of microRNAs in granulopoiesis.
- To elucidate the role of miR-15b in the proliferation and differentiation of acute promyelocytic leukemia cells.
- To identify the molecular targets and pathways regulated by miR-15b in APL.
Main Methods:
- Overexpression and knockdown experiments were performed on NB4 and HL60 cell lines.
- Identification of direct microRNA targets using molecular biology techniques.
- Analysis of cell cycle regulation and differentiation markers.
Main Results:
- miR-15b was found to inhibit proliferation and induce granulocytic differentiation in NB4 and HL60 cells.
- CCNE1 was identified as a direct target of miR-15b, playing a role in cell proliferation and differentiation.
- miR-15b promotes G0/G1 cell cycle arrest and terminal differentiation by targeting CCNE1 and modulating pRb in APL cells.
Conclusions:
- miR-15b plays a critical role in myeloid differentiation.
- The miR-15b/CCNE1 pathway regulates cell cycle arrest and promotes granulocyte differentiation in APL.
- miR-15b shows potential as a therapeutic agent for cancer treatment.
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